Methods of phenotypic identification of non-tuberculous mycobacteria

Non-tuberculous mycobacteria (NTM) are composed of mycobacterial species other than the Mycobacterium tuberculosis complex. Initially thought to be mere contaminants when isolated from clinical specimens, literature is increasing by the day showing NTM as proven pathogens. Due to the difference in a...

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Main Authors: Gurpreet S. Bhalla, Manbeer S. Sarao, Dinesh Kalra, Kuntal Bandyopadhyay, Arun Ravi John
Format: Article
Language:English
Published: Elsevier 2018-11-01
Series:Practical Laboratory Medicine
Online Access:http://www.sciencedirect.com/science/article/pii/S2352551717300823
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spelling doaj-055b24a282d94bdc8a433471e9535c682020-11-24T23:01:14ZengElsevierPractical Laboratory Medicine2352-55172018-11-0112Methods of phenotypic identification of non-tuberculous mycobacteriaGurpreet S. Bhalla0Manbeer S. Sarao1Dinesh Kalra2Kuntal Bandyopadhyay3Arun Ravi John4Department of Lab Sciences, Army Hospital (R&R), New Delhi 110010, India; Corresponding author.Div of Infectious Diseases, Detroit Medical Centre, Michigan, United StatesDepartment of Microbiology, Command Hospital (WC), Chandimandir, IndiaStation Health Officer, Amritsar, IndiaMedical Officer, Army Hospital (R&R), New Delhi, IndiaNon-tuberculous mycobacteria (NTM) are composed of mycobacterial species other than the Mycobacterium tuberculosis complex. Initially thought to be mere contaminants when isolated from clinical specimens, literature is increasing by the day showing NTM as proven pathogens. Due to the difference in antimicrobial susceptibility of different species, it becomes imperative for the microbiology laboratory to identify them to the species level. Molecular methods are available for rapid and accurate identification, but in a resource limited nation, phenotypic methods, albeit time consuming, are of paramount importance. By means of this article, the authors intend provide a concise summary of the basic biochemical reactions which can be done to identify most commonly isolated NTM. Keywords: Biochemical reactions, Non tuberculous mycobacteria, Phenotypic identificationhttp://www.sciencedirect.com/science/article/pii/S2352551717300823
collection DOAJ
language English
format Article
sources DOAJ
author Gurpreet S. Bhalla
Manbeer S. Sarao
Dinesh Kalra
Kuntal Bandyopadhyay
Arun Ravi John
spellingShingle Gurpreet S. Bhalla
Manbeer S. Sarao
Dinesh Kalra
Kuntal Bandyopadhyay
Arun Ravi John
Methods of phenotypic identification of non-tuberculous mycobacteria
Practical Laboratory Medicine
author_facet Gurpreet S. Bhalla
Manbeer S. Sarao
Dinesh Kalra
Kuntal Bandyopadhyay
Arun Ravi John
author_sort Gurpreet S. Bhalla
title Methods of phenotypic identification of non-tuberculous mycobacteria
title_short Methods of phenotypic identification of non-tuberculous mycobacteria
title_full Methods of phenotypic identification of non-tuberculous mycobacteria
title_fullStr Methods of phenotypic identification of non-tuberculous mycobacteria
title_full_unstemmed Methods of phenotypic identification of non-tuberculous mycobacteria
title_sort methods of phenotypic identification of non-tuberculous mycobacteria
publisher Elsevier
series Practical Laboratory Medicine
issn 2352-5517
publishDate 2018-11-01
description Non-tuberculous mycobacteria (NTM) are composed of mycobacterial species other than the Mycobacterium tuberculosis complex. Initially thought to be mere contaminants when isolated from clinical specimens, literature is increasing by the day showing NTM as proven pathogens. Due to the difference in antimicrobial susceptibility of different species, it becomes imperative for the microbiology laboratory to identify them to the species level. Molecular methods are available for rapid and accurate identification, but in a resource limited nation, phenotypic methods, albeit time consuming, are of paramount importance. By means of this article, the authors intend provide a concise summary of the basic biochemical reactions which can be done to identify most commonly isolated NTM. Keywords: Biochemical reactions, Non tuberculous mycobacteria, Phenotypic identification
url http://www.sciencedirect.com/science/article/pii/S2352551717300823
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