Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study

Background: Periodontal disease is an immune-inflammatory disease characterized by connective tissue breakdown, loss of attachment and alveolar bone resorption. In normal physiology, there is a dynamic equilibrium between reactive oxygen species activity and antioxidant defense capacity and when tha...

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Main Authors: Biju Thomas, Shabeer Mohamed Madani, B Rajendra Prasad, Suchetha Kumari
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2014-01-01
Series:Contemporary Clinical Dentistry
Subjects:
Online Access:http://www.contempclindent.org/article.asp?issn=0976-237X;year=2014;volume=5;issue=3;spage=340;epage=344;aulast=Thomas
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spelling doaj-0531e43739924bc3a559143a291781ef2020-11-24T23:23:47ZengWolters Kluwer Medknow PublicationsContemporary Clinical Dentistry0976-237X0976-23612014-01-015334034410.4103/0976-237X.137938Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional studyBiju ThomasShabeer Mohamed MadaniB Rajendra PrasadSuchetha KumariBackground: Periodontal disease is an immune-inflammatory disease characterized by connective tissue breakdown, loss of attachment and alveolar bone resorption. In normal physiology, there is a dynamic equilibrium between reactive oxygen species activity and antioxidant defense capacity and when that equilibrium shifts in favor of reactive oxygen species, oxidative stress results. Oxidative stress is thought to play a causative role in the pathogenesis of periodontal diseases. Catalase (CAT) protects cells from hydrogen peroxide generated within them. Even though, CAT is not essential for some cell types under normal conditions, it plays an important role countering the effects of oxidative stress on the cell. Aim: This study was designed to estimate and compare the CAT and total antioxidant capacity (TAOC) levels in the serum of periodontitis, gingivitis, and healthy individuals before and after nonsurgical periodontal therapy. Materials and Methods: This study was conducted in the Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore. The study was designed as a single blinded interventional study comprising of 75 subjects, inclusive of both sexes and divided into three groups of 25 patients each. Patients were categorized into chronic periodontitis, gingivitis and healthy. The severity of inflammation was assessed by using gingival index and pocket probing depth. Biochemical analysis was done to estimate the TAOC and CAT levels before and after nonsurgical periodontal therapy. Results obtained were then statistically analyzed using ANOVA test and paired t-test. Results: The results showed a higher level of serum TAOC and CAT in the healthy group compared with the other groups. The difference was found to be statistically significant (P < 0.0001). The posttreatment levels of TAOC were statistically higher than the pretreatment levels in periodontitis group.http://www.contempclindent.org/article.asp?issn=0976-237X;year=2014;volume=5;issue=3;spage=340;epage=344;aulast=ThomasAntioxidant defensecatalaseinterventional studyperiodontitistotal antioxidants
collection DOAJ
language English
format Article
sources DOAJ
author Biju Thomas
Shabeer Mohamed Madani
B Rajendra Prasad
Suchetha Kumari
spellingShingle Biju Thomas
Shabeer Mohamed Madani
B Rajendra Prasad
Suchetha Kumari
Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
Contemporary Clinical Dentistry
Antioxidant defense
catalase
interventional study
periodontitis
total antioxidants
author_facet Biju Thomas
Shabeer Mohamed Madani
B Rajendra Prasad
Suchetha Kumari
author_sort Biju Thomas
title Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
title_short Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
title_full Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
title_fullStr Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
title_full_unstemmed Comparative evaluation of serum antioxidant levels in periodontally diseased patients: An interventional study
title_sort comparative evaluation of serum antioxidant levels in periodontally diseased patients: an interventional study
publisher Wolters Kluwer Medknow Publications
series Contemporary Clinical Dentistry
issn 0976-237X
0976-2361
publishDate 2014-01-01
description Background: Periodontal disease is an immune-inflammatory disease characterized by connective tissue breakdown, loss of attachment and alveolar bone resorption. In normal physiology, there is a dynamic equilibrium between reactive oxygen species activity and antioxidant defense capacity and when that equilibrium shifts in favor of reactive oxygen species, oxidative stress results. Oxidative stress is thought to play a causative role in the pathogenesis of periodontal diseases. Catalase (CAT) protects cells from hydrogen peroxide generated within them. Even though, CAT is not essential for some cell types under normal conditions, it plays an important role countering the effects of oxidative stress on the cell. Aim: This study was designed to estimate and compare the CAT and total antioxidant capacity (TAOC) levels in the serum of periodontitis, gingivitis, and healthy individuals before and after nonsurgical periodontal therapy. Materials and Methods: This study was conducted in the Department of Periodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore. The study was designed as a single blinded interventional study comprising of 75 subjects, inclusive of both sexes and divided into three groups of 25 patients each. Patients were categorized into chronic periodontitis, gingivitis and healthy. The severity of inflammation was assessed by using gingival index and pocket probing depth. Biochemical analysis was done to estimate the TAOC and CAT levels before and after nonsurgical periodontal therapy. Results obtained were then statistically analyzed using ANOVA test and paired t-test. Results: The results showed a higher level of serum TAOC and CAT in the healthy group compared with the other groups. The difference was found to be statistically significant (P < 0.0001). The posttreatment levels of TAOC were statistically higher than the pretreatment levels in periodontitis group.
topic Antioxidant defense
catalase
interventional study
periodontitis
total antioxidants
url http://www.contempclindent.org/article.asp?issn=0976-237X;year=2014;volume=5;issue=3;spage=340;epage=344;aulast=Thomas
work_keys_str_mv AT bijuthomas comparativeevaluationofserumantioxidantlevelsinperiodontallydiseasedpatientsaninterventionalstudy
AT shabeermohamedmadani comparativeevaluationofserumantioxidantlevelsinperiodontallydiseasedpatientsaninterventionalstudy
AT brajendraprasad comparativeevaluationofserumantioxidantlevelsinperiodontallydiseasedpatientsaninterventionalstudy
AT suchethakumari comparativeevaluationofserumantioxidantlevelsinperiodontallydiseasedpatientsaninterventionalstudy
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