Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at...
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doaj-050dbca93de641bcb1cb2e2955402b872020-11-25T00:08:06ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942017-01-01201710.1155/2017/51403605140360Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary StudySylvia G. Lehmann0Sandrine Bourgoin-Voillard1Michel Seve2Walid Rachidi3University Grenoble Alpes, ISTerre, CS 40700, 38058 Grenoble Cedex 9, FranceUniversity Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, FranceUniversity Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, FranceUniversity Grenoble Alpes, INAC, SyMMES, 38000 Grenoble, FranceSkin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.http://dx.doi.org/10.1155/2017/5140360 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sylvia G. Lehmann Sandrine Bourgoin-Voillard Michel Seve Walid Rachidi |
spellingShingle |
Sylvia G. Lehmann Sandrine Bourgoin-Voillard Michel Seve Walid Rachidi Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study Oxidative Medicine and Cellular Longevity |
author_facet |
Sylvia G. Lehmann Sandrine Bourgoin-Voillard Michel Seve Walid Rachidi |
author_sort |
Sylvia G. Lehmann |
title |
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study |
title_short |
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study |
title_full |
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study |
title_fullStr |
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study |
title_full_unstemmed |
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study |
title_sort |
tubulin beta-3 chain as a new candidate protein biomarker of human skin aging: a preliminary study |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2017-01-01 |
description |
Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging. |
url |
http://dx.doi.org/10.1155/2017/5140360 |
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