Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study

Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at...

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Main Authors: Sylvia G. Lehmann, Sandrine Bourgoin-Voillard, Michel Seve, Walid Rachidi
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:Oxidative Medicine and Cellular Longevity
Online Access:http://dx.doi.org/10.1155/2017/5140360
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spelling doaj-050dbca93de641bcb1cb2e2955402b872020-11-25T00:08:06ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942017-01-01201710.1155/2017/51403605140360Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary StudySylvia G. Lehmann0Sandrine Bourgoin-Voillard1Michel Seve2Walid Rachidi3University Grenoble Alpes, ISTerre, CS 40700, 38058 Grenoble Cedex 9, FranceUniversity Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, FranceUniversity Grenoble Alpes, LBFA et BEeSy, PROMETHEE Proteomic Platform, Grenoble, FranceUniversity Grenoble Alpes, INAC, SyMMES, 38000 Grenoble, FranceSkin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.http://dx.doi.org/10.1155/2017/5140360
collection DOAJ
language English
format Article
sources DOAJ
author Sylvia G. Lehmann
Sandrine Bourgoin-Voillard
Michel Seve
Walid Rachidi
spellingShingle Sylvia G. Lehmann
Sandrine Bourgoin-Voillard
Michel Seve
Walid Rachidi
Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
Oxidative Medicine and Cellular Longevity
author_facet Sylvia G. Lehmann
Sandrine Bourgoin-Voillard
Michel Seve
Walid Rachidi
author_sort Sylvia G. Lehmann
title Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
title_short Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
title_full Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
title_fullStr Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
title_full_unstemmed Tubulin Beta-3 Chain as a New Candidate Protein Biomarker of Human Skin Aging: A Preliminary Study
title_sort tubulin beta-3 chain as a new candidate protein biomarker of human skin aging: a preliminary study
publisher Hindawi Limited
series Oxidative Medicine and Cellular Longevity
issn 1942-0900
1942-0994
publishDate 2017-01-01
description Skin aging is a complex process, and a lot of efforts have been made to identify new and specific targets that could help to diagnose, prevent, and treat skin aging. Several studies concerning skin aging have analyzed the changes in gene expression, and very few investigations have been performed at the protein level. Moreover, none of these proteomic studies has used a global quantitative labeled proteomic offgel approach that allows a more accurate description of aging phenotype. We applied such an approach on human primary keratinocytes obtained from sun-nonexposed skin biopsies of young and elderly women. A total of 517 unique proteins were identified, and 58 proteins were significantly differentially expressed with 40 that were downregulated and 18 upregulated with aging. Gene ontology and pathway analysis performed on these 58 putative biomarkers of skin aging evidenced that these dysregulated proteins were mostly involved in metabolism and cellular processes such as cell cycle and signaling pathways. Change of expression of tubulin beta-3 chain was confirmed by western blot on samples originated from several donors. Thus, this study suggested the tubulin beta-3 chain has a promising biomarker in skin aging.
url http://dx.doi.org/10.1155/2017/5140360
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