DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING

Staining impression smears from organ and tissues with peroxidase conjugated antibodies to Mycobacterium tuberculosis complex antigens, followed by visualization with diaminobenzidine and Kinyoun stains, ensured the painting of acid-resistant Mycobacterium tuberculosis forms to rubin red, acid-susce...

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Main Authors: A. P. Lysenko, V. V. Vlasenko, A. P. Lemish, T. P. Novik, E. A. Mikhalevich, I. G. Vlasenko
Format: Article
Language:Russian
Published: NEW TERRA Publishing House 2014-10-01
Series:Tuberkulez i Bolezni Lëgkih
Subjects:
Online Access:https://www.tibl-journal.com/jour/article/view/292
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spelling doaj-04dad8e9b9bf43c8821c2941342009552021-07-28T20:57:39ZrusNEW TERRA Publishing HouseTuberkulez i Bolezni Lëgkih2075-12302542-15062014-10-01010505410.21292/2075-1230-2014-0-10-50-54292DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAININGA. P. Lysenko0V. V. Vlasenko1A. P. Lemish2T. P. Novik3E. A. Mikhalevich4I. G. Vlasenko5Институт экспериментальной ветеринарии им. С. Н. Вышелесского, г. МинскВинницкий государственный аграрный университет, г. ВинницаИнститут экспериментальной ветеринарии им. С. Н. Вышелесского, г. МинскМинский государственный медицинский университет, г. МинскИнститут экспериментальной ветеринарии им. С. Н. Вышелесского, г. МинскВинницкий торгово-экономический институт Киевского национального торгово-экономического университета, г. ВинницаStaining impression smears from organ and tissues with peroxidase conjugated antibodies to Mycobacterium tuberculosis complex antigens, followed by visualization with diaminobenzidine and Kinyoun stains, ensured the painting of acid-resistant Mycobacterium tuberculosis forms to rubin red, acid-susceptible ones to brown, and tissue cells and microorganisms of other species to blue. Typical bacilli were absent in the lymph nodes of patients and animals with latent infection, but acid-resistant (rubin-red) granular forms were encountered in the granulomatous masses. Brown fat cells containing mycobacterial antigens, as well as acid-susceptible granular, reticular, fungoid, and rod-like forms were also found in considerable quantities.https://www.tibl-journal.com/jour/article/view/292mycobacterium tuberculosiscell wall-defective mycobacterium tuberculosisdifferentiating immunoperoxidase staining
collection DOAJ
language Russian
format Article
sources DOAJ
author A. P. Lysenko
V. V. Vlasenko
A. P. Lemish
T. P. Novik
E. A. Mikhalevich
I. G. Vlasenko
spellingShingle A. P. Lysenko
V. V. Vlasenko
A. P. Lemish
T. P. Novik
E. A. Mikhalevich
I. G. Vlasenko
DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
Tuberkulez i Bolezni Lëgkih
mycobacterium tuberculosis
cell wall-defective mycobacterium tuberculosis
differentiating immunoperoxidase staining
author_facet A. P. Lysenko
V. V. Vlasenko
A. P. Lemish
T. P. Novik
E. A. Mikhalevich
I. G. Vlasenko
author_sort A. P. Lysenko
title DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
title_short DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
title_full DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
title_fullStr DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
title_full_unstemmed DETECTION OF TISSUE MYCOBACTERIUM TUBERCULOSIS BY DIFFERENTIATING IMMUNOPEROXIDASE STAINING
title_sort detection of tissue mycobacterium tuberculosis by differentiating immunoperoxidase staining
publisher NEW TERRA Publishing House
series Tuberkulez i Bolezni Lëgkih
issn 2075-1230
2542-1506
publishDate 2014-10-01
description Staining impression smears from organ and tissues with peroxidase conjugated antibodies to Mycobacterium tuberculosis complex antigens, followed by visualization with diaminobenzidine and Kinyoun stains, ensured the painting of acid-resistant Mycobacterium tuberculosis forms to rubin red, acid-susceptible ones to brown, and tissue cells and microorganisms of other species to blue. Typical bacilli were absent in the lymph nodes of patients and animals with latent infection, but acid-resistant (rubin-red) granular forms were encountered in the granulomatous masses. Brown fat cells containing mycobacterial antigens, as well as acid-susceptible granular, reticular, fungoid, and rod-like forms were also found in considerable quantities.
topic mycobacterium tuberculosis
cell wall-defective mycobacterium tuberculosis
differentiating immunoperoxidase staining
url https://www.tibl-journal.com/jour/article/view/292
work_keys_str_mv AT aplysenko detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
AT vvvlasenko detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
AT aplemish detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
AT tpnovik detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
AT eamikhalevich detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
AT igvlasenko detectionoftissuemycobacteriumtuberculosisbydifferentiatingimmunoperoxidasestaining
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