Routine assay for determination of alpha-tocopherol in liver.
A rapid and accurate procedure for determination of alpha-tocopherol in liver has been developed. Extracts of saponified liver homogenates were purified by a simple and efficient chromatography step on Kieselgel. alpha-Tocopherol content of the purified extracts was determined by an automated gas–li...
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| Format: | Article |
| Language: | English |
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Elsevier
1980-08-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520348057 |
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doaj-04dad419cef84fa3b18a16ca6f4d01a52021-04-24T05:48:32ZengElsevierJournal of Lipid Research0022-22751980-08-01216780783Routine assay for determination of alpha-tocopherol in liver.A W KormannA rapid and accurate procedure for determination of alpha-tocopherol in liver has been developed. Extracts of saponified liver homogenates were purified by a simple and efficient chromatography step on Kieselgel. alpha-Tocopherol content of the purified extracts was determined by an automated gas–liquid chromatographic assay of the trimethylsilyl derivatives. Mean recovery for alpha-tocopherol added in vitro was 95.4 +/- 1.2% (SEM, 149 estimations). The lower assay limit was ca. 0.5 micrograms alpha-tocopherol/g liver. An experienced analyst was able to process at least 12 chick, rat, or geuinea pig livers per day.http://www.sciencedirect.com/science/article/pii/S0022227520348057 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
A W Kormann |
| spellingShingle |
A W Kormann Routine assay for determination of alpha-tocopherol in liver. Journal of Lipid Research |
| author_facet |
A W Kormann |
| author_sort |
A W Kormann |
| title |
Routine assay for determination of alpha-tocopherol in liver. |
| title_short |
Routine assay for determination of alpha-tocopherol in liver. |
| title_full |
Routine assay for determination of alpha-tocopherol in liver. |
| title_fullStr |
Routine assay for determination of alpha-tocopherol in liver. |
| title_full_unstemmed |
Routine assay for determination of alpha-tocopherol in liver. |
| title_sort |
routine assay for determination of alpha-tocopherol in liver. |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1980-08-01 |
| description |
A rapid and accurate procedure for determination of alpha-tocopherol in liver has been developed. Extracts of saponified liver homogenates were purified by a simple and efficient chromatography step on Kieselgel. alpha-Tocopherol content of the purified extracts was determined by an automated gas–liquid chromatographic assay of the trimethylsilyl derivatives. Mean recovery for alpha-tocopherol added in vitro was 95.4 +/- 1.2% (SEM, 149 estimations). The lower assay limit was ca. 0.5 micrograms alpha-tocopherol/g liver. An experienced analyst was able to process at least 12 chick, rat, or geuinea pig livers per day. |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520348057 |
| work_keys_str_mv |
AT awkormann routineassayfordeterminationofalphatocopherolinliver |
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