Routine assay for determination of alpha-tocopherol in liver.

A rapid and accurate procedure for determination of alpha-tocopherol in liver has been developed. Extracts of saponified liver homogenates were purified by a simple and efficient chromatography step on Kieselgel. alpha-Tocopherol content of the purified extracts was determined by an automated gas–li...

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Bibliographic Details
Main Author: A W Kormann
Format: Article
Language:English
Published: Elsevier 1980-08-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520348057
Description
Summary:A rapid and accurate procedure for determination of alpha-tocopherol in liver has been developed. Extracts of saponified liver homogenates were purified by a simple and efficient chromatography step on Kieselgel. alpha-Tocopherol content of the purified extracts was determined by an automated gas–liquid chromatographic assay of the trimethylsilyl derivatives. Mean recovery for alpha-tocopherol added in vitro was 95.4 +/- 1.2% (SEM, 149 estimations). The lower assay limit was ca. 0.5 micrograms alpha-tocopherol/g liver. An experienced analyst was able to process at least 12 chick, rat, or geuinea pig livers per day.
ISSN:0022-2275