Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells

Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells

Fibrillar accumulation of A53T mutant α-synuclein (A53T-AS) in Lewy bodies is a symptom of Parkinsonism. Inhibitions of the overexpression and fibrillar aggregation of α-synuclein (AS) in vivo could be a promising strategy for treating Parkinson’s disease (PD). In this study, at concentrations lower...

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Main Authors: Juan Zhao, Xiuling Zhi, Luanfeng Pan, Ping Zhou
Format: Article
Language:English
Published: MDPI AG 2017-08-01
Series:Molecules
Subjects:
α-synuclein
trehalose
transduced PC12 cell
Parkinson’s disease
Online Access:https://www.mdpi.com/1420-3049/22/8/1293
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spelling doaj-04c9786b9560408b8ac0b65e4faa7ce62020-11-24T22:52:28ZengMDPI AGMolecules1420-30492017-08-01228129310.3390/molecules22081293molecules22081293Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 CellsJuan Zhao0Xiuling Zhi1Luanfeng Pan2Ping Zhou3State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaLaboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaLaboratory of Molecular Biology, Shanghai Medical College, Fudan University, Shanghai 200032, ChinaState Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, ChinaFibrillar accumulation of A53T mutant α-synuclein (A53T-AS) in Lewy bodies is a symptom of Parkinsonism. Inhibitions of the overexpression and fibrillar aggregation of α-synuclein (AS) in vivo could be a promising strategy for treating Parkinson’s disease (PD). In this study, at concentrations lower than 1 mM, trehalose decreased the A53T-AS expression level in transduced PC12 cells. Although H2O2 and aluminum ions increased the expression level and neurotoxicity of A53T-AS in cells, proper trehalose concentrations inhibited the event. These studies adequately prove that trehalose at an appropriate dose would be potentially useful for PD treatment.https://www.mdpi.com/1420-3049/22/8/1293α-synucleintrehalosetransduced PC12 cellParkinson’s disease
collection DOAJ
language English
format Article
sources DOAJ
author Juan Zhao
Xiuling Zhi
Luanfeng Pan
Ping Zhou
spellingShingle Juan Zhao
Xiuling Zhi
Luanfeng Pan
Ping Zhou
Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
Molecules
α-synuclein
trehalose
transduced PC12 cell
Parkinson’s disease
author_facet Juan Zhao
Xiuling Zhi
Luanfeng Pan
Ping Zhou
author_sort Juan Zhao
title Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
title_short Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
title_full Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
title_fullStr Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
title_full_unstemmed Trehalose Inhibits A53T Mutant α-Synuclein Overexpression and Neurotoxicity in Transduced PC12 Cells
title_sort trehalose inhibits a53t mutant α-synuclein overexpression and neurotoxicity in transduced pc12 cells
publisher MDPI AG
series Molecules
issn 1420-3049
publishDate 2017-08-01
description Fibrillar accumulation of A53T mutant α-synuclein (A53T-AS) in Lewy bodies is a symptom of Parkinsonism. Inhibitions of the overexpression and fibrillar aggregation of α-synuclein (AS) in vivo could be a promising strategy for treating Parkinson’s disease (PD). In this study, at concentrations lower than 1 mM, trehalose decreased the A53T-AS expression level in transduced PC12 cells. Although H2O2 and aluminum ions increased the expression level and neurotoxicity of A53T-AS in cells, proper trehalose concentrations inhibited the event. These studies adequately prove that trehalose at an appropriate dose would be potentially useful for PD treatment.
topic α-synuclein
trehalose
transduced PC12 cell
Parkinson’s disease
url https://www.mdpi.com/1420-3049/22/8/1293
work_keys_str_mv AT juanzhao trehaloseinhibitsa53tmutantasynucleinoverexpressionandneurotoxicityintransducedpc12cells
AT xiulingzhi trehaloseinhibitsa53tmutantasynucleinoverexpressionandneurotoxicityintransducedpc12cells
AT luanfengpan trehaloseinhibitsa53tmutantasynucleinoverexpressionandneurotoxicityintransducedpc12cells
AT pingzhou trehaloseinhibitsa53tmutantasynucleinoverexpressionandneurotoxicityintransducedpc12cells
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