Typification of the staphylococcal chromosome cassette of methicillin-resistant Staphylococcus aureus in the State of Aragua, Venezuela

• Main Authors: Betsi Bastidas, María V. Méndez, Ysvette Vásquez, Dayana Requena
• Format: Article
• Language: Spanish
• Published: Instituto Nacional de Salud 2020-06-01
• Series: Revista Peruana de Medicina Experimental y Salud Pública
• Subjects: staphylococcus aureus; sccmec; gen meca; epidemiología molecular; staphylococcus aureus resistentes a meticilina
• Online Access: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4652

Objective: Typify the SCCmec cassette in methicillin-resistant strains of Staphylococcus aureus in clinical isolates from health centers in the State of Aragua-Venezuela and compare the presence of SCCmec genotypes among the state health centers and according to the type of infection. Materials and methods: 81 MRSA strains from four health centers of the Aragua-Venezuela State were studied. Methicillin resistance was performed with the Kirby-Bauer method with oxacillin (1 μg) and cefoxitin (30 μg) disks. The mecA gene and SCCmec were analyzed by the multiple PCR technique. Results: Only 55 isolates (67.9%) amplified the mecA gene, and 24 strains (43.6%) amplified SCCmec. SCCmec type I was the most frequency, followed by SCCmec IV and SCCmec III, representing 62.5%, 25% and 12.5%, respectively. SCCmec I was predominant in health center A (80%), while in B and C 60% and 100% respectively were SCCmec IV. At health center D, 50% turned out to be SCCmec I and 50% SCCmec IVd. A relationship was found between the SCCmec and the health center with statistical significance. SCCmec I predominated in skin and soft tissue and respiratory infections with 63.2% and 50%, respectively. There was no association between genotype and type of infection with a p value greater than 0.05. Conclusions: The prevalence of SCCmec I and IV will allow establishing new measures in the use of antibiotics and epidemiological control.