PROSPECTS OF USE OF RICE VARIETY WITH GENE SUB1 TOLERANT TO DEEPWATER IN THE BREEDING OF RUSSIAN VARIETIES

• Main Authors: P. I. Kostylev, A. A. Redkin, E. V. Krasnova, A. V. Usatov, M. S. Makarenko
• Format: Article
• Language: Russian
• Published: Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" 2018-05-01
• Series: Зерновое хозяйство России
• Subjects: rice; hybrid; donor; tolerance (resistance) to deep watering; molecular marker; pcr-analysis
• Online Access: https://www.zhros.online/jour/article/view/183
• ISSN: 2079-8725; 2079-8733

Growing of varieties tolerant to deepwater during germination is an effective method of rice protection from weeds without use of herbicides. To develop such varieties it’s necessary to combine genes of great energy of early germination, genes resistant to anaerobic germination, deepwater and lodging into one geno type.  Identification of molecular markers, connected with genes resistant to these stresses, makes breeding work easier. That’s why development of rice varieties with the help of marking is of great importance for non-herbicide technologies. The locus Sub1 regulates response to ethylene and gibberellin which results in reduce of carbohydrates consumption and sprouts’ peace under water and promotes tolerance to deep watering. As donors of resistant genes Sub1 we used such varieties with the gene Sub1 as ‘BR-11’, ‘CR-1009’, ‘Inbara-3’ and ‘TDК-1’. As a recipient we took an early maturing variety ‘Novator’. We also used micro satellite markers on the gene Sub1. The identification of the gene Sub1 was carried out by the method of molecular marking with the use of specific primers on the basis of PCR. During the work in 2013-2014 we carried out hybridization and received hybrids F1-F2 of the variety ‘Novator’ with four Asian rice varieties carrying the gene Sub1.  Due to studies with the help of present biotechnologies together with traditional breeding methods we developed early maturing rice varieties with the gene Sub1, suitable for growing in the Rostov region.