RNA Profiling for Biomarker Discovery: Practical Considerations for Limiting Sample Sizes
We have compared microarray data generated on Affymetrix™ chips from standard (8 micrograms) or low (100 nanograms) amounts of total RNA. We evaluated the gene signals and gene fold-change estimates obtained from the two methods and validated a subset of the results by real time, polymerase chain re...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2005-01-01
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| Series: | Disease Markers |
| Online Access: | http://dx.doi.org/10.1155/2005/357089 |