CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene

SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which...

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Main Authors:	Zhi-Bin Qiao, Ji-Zhen Lu, Lu Zhang, Hong-Xia Cao, Zhi-Hui Bai, Yi-Yao Qi, Han-Yu Zhu, Shou-Mei Zhang, Yan Bao, Wen-Wen Jia, Zhong-Min Liu
Format:	Article
Language:	English
Published:	Elsevier 2021-10-01
Series:	Stem Cell Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S1873506121003664

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spelling	doaj-046332ec6b5c4e4093d5785cb247f21a2021-08-30T04:13:16ZengElsevierStem Cell Research1873-50612021-10-0156102519CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 geneZhi-Bin Qiao0Ji-Zhen Lu1Lu Zhang2Hong-Xia Cao3Zhi-Hui Bai4Yi-Yao Qi5Han-Yu Zhu6Shou-Mei Zhang7Yan Bao8Wen-Wen Jia9Zhong-Min Liu10Institute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaInstitute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaCorresponding authors.; Institute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaCorresponding authors.; Institute for Regenerative Medicine, National Stem Cell Translational Resource Center, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, ChinaSUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above.http://www.sciencedirect.com/science/article/pii/S1873506121003664
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Zhi-Bin Qiao Ji-Zhen Lu Lu Zhang Hong-Xia Cao Zhi-Hui Bai Yi-Yao Qi Han-Yu Zhu Shou-Mei Zhang Yan Bao Wen-Wen Jia Zhong-Min Liu
spellingShingle	Zhi-Bin Qiao Ji-Zhen Lu Lu Zhang Hong-Xia Cao Zhi-Hui Bai Yi-Yao Qi Han-Yu Zhu Shou-Mei Zhang Yan Bao Wen-Wen Jia Zhong-Min Liu CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene Stem Cell Research
author_facet	Zhi-Bin Qiao Ji-Zhen Lu Lu Zhang Hong-Xia Cao Zhi-Hui Bai Yi-Yao Qi Han-Yu Zhu Shou-Mei Zhang Yan Bao Wen-Wen Jia Zhong-Min Liu
author_sort	Zhi-Bin Qiao
title	CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
title_short	CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
title_full	CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
title_fullStr	CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
title_full_unstemmed	CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene
title_sort	crispr/cas9 mediated generation of a ipsc line ehtjui005-a-1 with homozygous knockout of the suv39h1 gene
publisher	Elsevier
series	Stem Cell Research
issn	1873-5061
publishDate	2021-10-01
description	SUV39H1 is a histone methyltransferase involve numerous biological processes, including of aging, embryo development, tumor growth and mitosis via catalysis of dimethylation and trimethylation of lysine 9 of histone H3. Here we report a human induced pluripotent stem cell line (EHTJUi005-A-1) which is generated from a wildtype human iPSC previously established in our laboratory, and this iPSC has a homozygous knockout of 8 bp in Exon 2 of SUV39H1. This iPSC model provides a valuable resource to study epigenetic regulation in extensive biological processes as mentioned above.
url	http://www.sciencedirect.com/science/article/pii/S1873506121003664
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CRISPR/Cas9 mediated generation of a iPSC line EHTJUi005-A-1 with homozygous knockout of the SUV39H1 gene

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