PROXIMAL COMPOSITION AND ANTIOXIDANT ACTIVITY OF THE EXTRACT OF PASSION FRUIT SEEDS (Passiflora edulis) IN SOYBEAN OIL COMPOSIÇÃO CENTESIMAL E ATIVIDADE ANTIOXIDANTE DO EXTRATO DE SEMENTES DE MARACUJÁ (Passiflora edulis) EM ÓLEO DE SOJA

• Main Authors: Priscila Milene Angelo, Cassia Roberta Malacrida, Neuza Jorge, Denise Andreo
• Format: Article
• Language: English
• Published: Universidade Federal de Goiás 2009-10-01
• Series: Pesquisa Agropecuária Tropical
• Subjects: Antioxidantes; sementes de maracujá; índice de peróxidos; composição centesimal
• Online Access: http://www.revistas.ufg.br/index.php/pat/article/view/3566

&lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;This study evaluated the antioxidant activity of passion fruit seeds extract in soybean oil. Dried seeds were ground and submitted to extraction with ethanol:water (95:5), for two hours, at room temperature. The resulting extract was centrifuged and the supernatant evaporated for dry extract yield calculation. The extract of passion fruit seeds was suspended again in ethanol:water (95:5) and applied to soybean oil in two concentrations (500 mg kg&lt;sup&gt;-1&lt;/sup&gt; and 1,000 mg kg&lt;sup&gt;-1&lt;/sup&gt;), being submitted to oven accelerated test at 60&lt;sup&gt;o&lt;/sup&gt;C, for 20 days. Oil samples were taken every 5 days and analyzed for peroxide value. As a parameter of comparison, it was used butyl hydroxytoluene (BHT) as well as refined soybean oil without antioxidants (control). In the extract, the antioxidant activity was measured by the DPPH radical&lt;span style="color: #ff0000;"&gt; &lt;/span&gt;method and total phenols by using the Folin-Ciocalteau method. The yield of dry extract was 1.26%, the IC&lt;sub&gt;50&lt;/sub&gt; value was 113.41 µg mL&lt;sup&gt;-1&lt;/sup&gt;, and the concentration of total phenolic compounds was 42.93 mg of gallic acid per gram of extract. At the final heating time, peroxide values were significantly different and the antioxidant activity order was: BHT &gt; passion fruit 500 mg kg&lt;sup&gt;-1&lt;/sup&gt; &gt; passion fruit 1,000 mg kg&lt;sup&gt;-1&lt;/sup&gt; &gt; control.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;KEY-WORDS: Antioxidants; passion fruit seeds; peroxide value; proximal composition.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;<br>&lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;Este trabalho consistiu em avaliar a atividade antioxidante do extrato de sementes de maracujá em óleo de soja. As sementes desidratadas foram trituradas e submetidas à extração em etanol:água (95:5), por duas horas, à temperatura ambiente. O extrato resultante foi centrifugado e o sobrenadante rotoevaporado, a fim de se calcular o rendimento de extrato seco. O extrato de sementes de maracujá foi ressuspenso no mesmo solvente utilizado para a extração e aplicado no óleo de soja, em duas concentrações (500 mg kg&lt;sup&gt;-1&lt;/sup&gt; e 1.000 mg kg&lt;sup&gt;-1&lt;/sup&gt;), sendo submetido ao teste acelerado de estufa, a 60ºC, por 20 dias. Amostras de óleo foram retiradas a cada 5 dias e analisadas quanto ao índice de peróxidos. Como parâmetro de comparação, foi utilizado butil hidroxitolueno (BHT), bem como o óleo de soja refinado, isento de antioxidantes (controle). No extrato, a atividade antioxidante foi mensurada pelo método do radical DPPH&lt;span style="color: #ff0000;"&gt; &lt;/span&gt;e a concentração de compostos fenólicos totais pelo método de Folin-Ciocalteau. O rendimento em extrato seco foi 1,26%, o valor de CE&lt;sub&gt;50&lt;/sub&gt; encontrado foi 113,41 µg mL&lt;sup&gt;-1&lt;/sup&gt; e a concentração de compostos fenólicos totais foi 42,93 mg de ácido gálico/g de extrato. Para o tempo final de estufa, os valores de peróxidos diferiram significativamente entre si e a ordem de atividade antioxidante foi: BHT &gt; maracujá 500 mg kg&lt;sup&gt;-1&lt;/sup&gt; &gt; maracujá 1.000 mg kg&lt;sup&gt;-1&lt;/sup&gt; &gt; controle.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;PALAVRAS-CHAVE: Antioxidantes; sementes de maracujá; índice de peróxidos; composição centesimal.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;