Morphological and Genotypic Variations among the Species of the Subgenus Adlerius (Diptera: Psychodidae, Phlebotomus) in Iran

• Main Authors: Alireza Zahraei-Ramazani, Dinesh Kumar, Hossein Mirhendi, Shyam Sundar, Rajnikan Mishra, Vahideh Moin-Vaziri, Hassan Soleimani, MohammadReza Shirzadi, Reza Jafari, AhmadAli Hanafi-Bojd, Sodabe Hamedi-Shahraky, MohammadReza Yaghoobi-Ershadi
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2015-10-01
• Series: Journal of Arthropod-Borne Diseases
• Subjects: Phlebotomine sand flies; subgenus Adlerius; P. comatus; P. kabulensis; Mitochondrial DNA Cytochrome b gene
• Online Access: https://jad.tums.ac.ir/index.php/jad/article/view/120
• ISSN: 1735-7179; 2322-2271

Background: Female sand flies of subgenus Adlerius are considered as probable vectors of visceral leishmaniasis in Iran. The objective of this study was to determine the morphological and genotypic variations in the populations of this subgenus in the country. Methods: Sand flies collected using sticky traps from 17 provinces during 2008–2010. The morphometric measurements were conducted with an Ocular Micrometer. Data was analyzed by SPSS. The Cytb gene was used to estimate population genetic diversity and identify the female specimens. UPGMA phenetic tree was used for DNA haplotypes of Cytb gene. Results: Six species of subgenus Adlerius identified from which one species, P. (Adlerius) kabulensis, is new record. The identification key is provided for males. Results revealed the molecular systematic in the species of subgenus Adlerius and determine the relationship of three females of P. comatus, P. balcanicus and P. halepensis. Conclusion: The positions of three females and the males in the UPGMA tree are correct and the similarities among them confirm our results. The branches of each species are not genetically distinct which justify the overlapping morphological characters among them. Molecular sequencing of Cytb-mtDNA haplotypes can be used for female identification for different species of subgenus Adlerius in Iran.