Angiostrongylus costaricensis and experimental infection of Sarasinula marginata II: elimination routes

• Main Authors: Mendonça Cristiane Lafeta Gomes Furtado, Carvalho Omar Santos, Mota Ester Maria, Pelajo-Machado Marcelo, Caputo Luzia Fátima Gonçalves, Lenzi Henrique Leonel
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003-01-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Angiostrongylus costaricensis; Sarasinula marginata; Veronicellidae; migratory routes; larval elimination; amebocyte; invertebrate granuloma
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700006
• ISSN: 0074-0276; 1678-8060

Angiostrongylus costaricensis intermediate hosts are terrestrial mollusks mostly belonging to the Veronicellidae family. In the present investigation we focused on the mechanisms of larval expulsion from Sarasinula marginata infected with A. costaricensis. Twenty-five mollusks were individually infected with 5000 L1 and sacrificed at 30 min and 1, 2, 4, 6, and 8 h post-infection and at days 1, 2, 4, 5, 6, 8, 10, 11, 12, 14, 15, 16, 20, 21, 22, 25, 26, 28, and 30 post-infection; the mollusks were then fixed and stained. Diverse organs involved throughout the course of the migratory routes of larvae from oral penetration on were specified and the mechanisms of larval access to the fibromuscular layer through the kidney, rectum, and vascular system were defined. The elimination of L3, derived from oral and/or cutaneous infections, appears to depend on granulomas located close to the excretory ducts of mucous cells.