ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS

PURPOSE. The purpose of this work was to evaluate the content of Lactobacillus bulgaricus in faecal samples of volunteers after consumption of yoghurt produced with selected L. bulgaricus strain. METHODS. Using Denaturation Gradient Gel Electrophoresis (DGGE) the DNA band corresponding to L. bulgari...

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Main Author: Zh. Dimitrov
Format: Article
Language:English
Published: Trakia University 2019-12-01
Series:Trakia Journal of Sciences
Subjects:
Online Access:http://tru.uni-sz.bg/tsj/vol17,sp4-2019/3.pdf
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spelling doaj-0430e870d50b4b3d817145b283a64aec2021-04-02T06:05:38ZengTrakia UniversityTrakia Journal of Sciences1312-17231313-35512019-12-0117431231710.15547/tjs.2019.04.003ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESISZh. DimitrovPURPOSE. The purpose of this work was to evaluate the content of Lactobacillus bulgaricus in faecal samples of volunteers after consumption of yoghurt produced with selected L. bulgaricus strain. METHODS. Using Denaturation Gradient Gel Electrophoresis (DGGE) the DNA band corresponding to L. bulgaricus was well separated from the DNA bands of other lactobacilli. RESULTS. Faecal samples of all four volunteers consumed yoghurt contained a major DNA band indicating the presence of L. bulgaricus, unlike the faecal samples from the three volunteers no consumed yoghurt where the specific for L. bulgaricus DNA band was absent. The differences in the intensity of the electrophoretic band of L. bulgaricus are influenced by the individuality of the volunteers and the duration of yoghurt consumption. By help of DGGE it was possible to assay the presence of L. bulgaricus in faecal samples and on the base of the intensity of the specific DNA fragment to evaluate the dynamics of L. bulgaricus content during 10 days consumption of yoghurt. CONCLUSIONS. DGGE proved to be an applicable tool to monitor the presence of L. bulgaricus strains in human intestinal samples.http://tru.uni-sz.bg/tsj/vol17,sp4-2019/3.pdffaecal microfloramicrobiotamicrobial communities
collection DOAJ
language English
format Article
sources DOAJ
author Zh. Dimitrov
spellingShingle Zh. Dimitrov
ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
Trakia Journal of Sciences
faecal microflora
microbiota
microbial communities
author_facet Zh. Dimitrov
author_sort Zh. Dimitrov
title ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
title_short ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
title_full ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
title_fullStr ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
title_full_unstemmed ASSESSMENT OF LACTOBACILLUS BULGARICUS STRAIN IN HUMAN INTESTINAL SAMPLES BY DENATURATION GRADIENT GEL ELECTROPHORESIS
title_sort assessment of lactobacillus bulgaricus strain in human intestinal samples by denaturation gradient gel electrophoresis
publisher Trakia University
series Trakia Journal of Sciences
issn 1312-1723
1313-3551
publishDate 2019-12-01
description PURPOSE. The purpose of this work was to evaluate the content of Lactobacillus bulgaricus in faecal samples of volunteers after consumption of yoghurt produced with selected L. bulgaricus strain. METHODS. Using Denaturation Gradient Gel Electrophoresis (DGGE) the DNA band corresponding to L. bulgaricus was well separated from the DNA bands of other lactobacilli. RESULTS. Faecal samples of all four volunteers consumed yoghurt contained a major DNA band indicating the presence of L. bulgaricus, unlike the faecal samples from the three volunteers no consumed yoghurt where the specific for L. bulgaricus DNA band was absent. The differences in the intensity of the electrophoretic band of L. bulgaricus are influenced by the individuality of the volunteers and the duration of yoghurt consumption. By help of DGGE it was possible to assay the presence of L. bulgaricus in faecal samples and on the base of the intensity of the specific DNA fragment to evaluate the dynamics of L. bulgaricus content during 10 days consumption of yoghurt. CONCLUSIONS. DGGE proved to be an applicable tool to monitor the presence of L. bulgaricus strains in human intestinal samples.
topic faecal microflora
microbiota
microbial communities
url http://tru.uni-sz.bg/tsj/vol17,sp4-2019/3.pdf
work_keys_str_mv AT zhdimitrov assessmentoflactobacillusbulgaricusstraininhumanintestinalsamplesbydenaturationgradientgelelectrophoresis
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