miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma

Background: Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions. Notch signaling is a conserved evolu...

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Main Authors: Zahra Asefy, Sirus Hoseinnejhad, Sanam Dolati, Zaker Ceferov, Amir Hasanzadeh, Robab Azergun, Mohammad Nouri
Format: Article
Language:English
Published: Hamadan University of Medical Sciences 2019-06-01
Series:Avicenna Journal of Medical Biochemistry
Subjects:
Online Access:http://ajmb.umsha.ac.ir/PDF/ajmb-2165
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spelling doaj-041129d5a4c745af88f58d22ca0cfa2a2021-08-02T22:08:58ZengHamadan University of Medical SciencesAvicenna Journal of Medical Biochemistry2345-41132019-06-01714810.34172/ajmb.2019.02ajmb-2165miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular CarcinomaZahra Asefy0Sirus Hoseinnejhad1Sanam Dolati2Zaker Ceferov3Amir Hasanzadeh4Robab Azergun5Mohammad Nouri6Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Biochemistry, Baku State University, Baku, Azerbaijan.Medicine Faculty (Biochemistry, Immunology, Microbiology Departments), Tabriz University of Medical Sciences, Tabriz, Iran.Department of Biochemistry, Baku State University, Baku, Azerbaijan.Maragheh University of Medical Sciences, Maragheh, Iran.Maragheh University of Medical Sciences, Maragheh, Iran.Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Background: Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions. Notch signaling is a conserved evolutionary pathway that controls cell functions. The dysregulation of this pathway leads to different diseases such as cancer. Objectives: This study aimed to investigate the role of miR-515-5p and Notch1 as new diagnostic markers in HCC. Methods: Forty formalin fixed paraffin embedded (FFPE) autopsy blocks and 40 FFPE normal liver tissues were selected from the archives of the pathology of Imam Reza hospital, Tabriz, Iran. Real-time polymerase chain reaction (PCR) was used for gene expression. Immune histochemistry method was used for detecting notch1 in normal and cancer FFPE tissues. Hematoxylin and eosin staining was also used for the diagnosis of normal and cancerous tissues. Results: miR-515-5P showed higher expression in the cancer group compared to the normal group (4.7 fold). Hematoxylin and eosin staining of HCC tissues showed significant color intensity than that of normal tissues. Immune histochemistry results revealed significant Ag-Ab reaction in the cancer group. In this study, we analyzed miRNA gene expression and notch 1 level in HCC patients. miRNA dysregulation has been found in a large variety of HCCs. Hepatocarcinogenesis was associated with the expression level of miR-515-5p with carcinogenesis. Moreover, notch1 was a key protein in liver cell fate and a progressive molecule in HCC. Conclusion: Our study demonstrated the main role of miR-515-5p in the pathogenesis of HCC. Likewise, it disclosed the expression of these genes could be utilized in HCC prognosis.http://ajmb.umsha.ac.ir/PDF/ajmb-2165biomarkerneoplasmnotch1 proteinmicrorna
collection DOAJ
language English
format Article
sources DOAJ
author Zahra Asefy
Sirus Hoseinnejhad
Sanam Dolati
Zaker Ceferov
Amir Hasanzadeh
Robab Azergun
Mohammad Nouri
spellingShingle Zahra Asefy
Sirus Hoseinnejhad
Sanam Dolati
Zaker Ceferov
Amir Hasanzadeh
Robab Azergun
Mohammad Nouri
miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
Avicenna Journal of Medical Biochemistry
biomarker
neoplasm
notch1 protein
microrna
author_facet Zahra Asefy
Sirus Hoseinnejhad
Sanam Dolati
Zaker Ceferov
Amir Hasanzadeh
Robab Azergun
Mohammad Nouri
author_sort Zahra Asefy
title miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
title_short miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
title_full miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
title_fullStr miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
title_full_unstemmed miR-515-5p and Notch1 as New Diagnostic Markers of Hepatocellular Carcinoma
title_sort mir-515-5p and notch1 as new diagnostic markers of hepatocellular carcinoma
publisher Hamadan University of Medical Sciences
series Avicenna Journal of Medical Biochemistry
issn 2345-4113
publishDate 2019-06-01
description Background: Modifications of miRNA expression have been related to various types of cancers including hepatocellular carcinoma (HCC). miRNAs directly act as repressors of gene expression, as they reside in fragile sites, as well as cancer-related genomic regions. Notch signaling is a conserved evolutionary pathway that controls cell functions. The dysregulation of this pathway leads to different diseases such as cancer. Objectives: This study aimed to investigate the role of miR-515-5p and Notch1 as new diagnostic markers in HCC. Methods: Forty formalin fixed paraffin embedded (FFPE) autopsy blocks and 40 FFPE normal liver tissues were selected from the archives of the pathology of Imam Reza hospital, Tabriz, Iran. Real-time polymerase chain reaction (PCR) was used for gene expression. Immune histochemistry method was used for detecting notch1 in normal and cancer FFPE tissues. Hematoxylin and eosin staining was also used for the diagnosis of normal and cancerous tissues. Results: miR-515-5P showed higher expression in the cancer group compared to the normal group (4.7 fold). Hematoxylin and eosin staining of HCC tissues showed significant color intensity than that of normal tissues. Immune histochemistry results revealed significant Ag-Ab reaction in the cancer group. In this study, we analyzed miRNA gene expression and notch 1 level in HCC patients. miRNA dysregulation has been found in a large variety of HCCs. Hepatocarcinogenesis was associated with the expression level of miR-515-5p with carcinogenesis. Moreover, notch1 was a key protein in liver cell fate and a progressive molecule in HCC. Conclusion: Our study demonstrated the main role of miR-515-5p in the pathogenesis of HCC. Likewise, it disclosed the expression of these genes could be utilized in HCC prognosis.
topic biomarker
neoplasm
notch1 protein
microrna
url http://ajmb.umsha.ac.ir/PDF/ajmb-2165
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