Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation
A procedure is described for the separation of plasma Sf > 400 and Sf 20-400 lipoproteins each into three fractions. Serum samples are overlayered with a sodium chloride density gradient in a preparative ultracentrifuge tube and thin layers are removed at the top of the tube after successive cent...
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Elsevier
1969-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520426501 |
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doaj-03fd05250fb341559802a982bbbea5df2021-04-24T05:54:22ZengElsevierJournal of Lipid Research0022-22751969-01-011016876Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugationW.J. Lossow0F.T. Lindgren1J.C. Murchio2G.R. Stevens3L.C. Jensen4Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720; Air and Industrial Hygiene Laboratory, Division of Laboratories, California State Department of Public Health, Berkeley, California 94704Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720; Air and Industrial Hygiene Laboratory, Division of Laboratories, California State Department of Public Health, Berkeley, California 94704Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720; Air and Industrial Hygiene Laboratory, Division of Laboratories, California State Department of Public Health, Berkeley, California 94704Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720; Air and Industrial Hygiene Laboratory, Division of Laboratories, California State Department of Public Health, Berkeley, California 94704Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720; Air and Industrial Hygiene Laboratory, Division of Laboratories, California State Department of Public Health, Berkeley, California 94704A procedure is described for the separation of plasma Sf > 400 and Sf 20-400 lipoproteins each into three fractions. Serum samples are overlayered with a sodium chloride density gradient in a preparative ultracentrifuge tube and thin layers are removed at the top of the tube after successive centrifugations at different speeds in a swinging bucket rotor.The procedure was evaluated by electron microscopy of the Sf > 400 lipoprotein fractions and schlieren analysis of the Sf 20-400 lipoprotein fractions. Protein content of each fraction was measured by elemental N, C, H, and lipid-P analysis. Protein coverage was calculated for all fractions on the assumption that there is a surface layer 20 A thick.For the entire Sf > 400 lipoprotein spectrum and for a part of the Sf 20-400 lipoprotein distribution the proportion of surface covered by protein was constant (approximately 20% coverage). Therefore, for these portions of the lipoprotein spectrum, the increase in surface: volume ratio as particle size decreases is approximately compensated for by an increase in the weight percentage of protein.http://www.sciencedirect.com/science/article/pii/S0022227520426501chylomicronsvery low density lipoproteinselectron microscopyanalytical centrifugationprotein coverage |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
W.J. Lossow F.T. Lindgren J.C. Murchio G.R. Stevens L.C. Jensen |
| spellingShingle |
W.J. Lossow F.T. Lindgren J.C. Murchio G.R. Stevens L.C. Jensen Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation Journal of Lipid Research chylomicrons very low density lipoproteins electron microscopy analytical centrifugation protein coverage |
| author_facet |
W.J. Lossow F.T. Lindgren J.C. Murchio G.R. Stevens L.C. Jensen |
| author_sort |
W.J. Lossow |
| title |
Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| title_short |
Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| title_full |
Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| title_fullStr |
Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| title_full_unstemmed |
Particle size and protein content of six fractions of the Sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| title_sort |
particle size and protein content of six fractions of the sf > 20 plasma lipoproteins isolated by density gradient centrifugation |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1969-01-01 |
| description |
A procedure is described for the separation of plasma Sf > 400 and Sf 20-400 lipoproteins each into three fractions. Serum samples are overlayered with a sodium chloride density gradient in a preparative ultracentrifuge tube and thin layers are removed at the top of the tube after successive centrifugations at different speeds in a swinging bucket rotor.The procedure was evaluated by electron microscopy of the Sf > 400 lipoprotein fractions and schlieren analysis of the Sf 20-400 lipoprotein fractions. Protein content of each fraction was measured by elemental N, C, H, and lipid-P analysis. Protein coverage was calculated for all fractions on the assumption that there is a surface layer 20 A thick.For the entire Sf > 400 lipoprotein spectrum and for a part of the Sf 20-400 lipoprotein distribution the proportion of surface covered by protein was constant (approximately 20% coverage). Therefore, for these portions of the lipoprotein spectrum, the increase in surface: volume ratio as particle size decreases is approximately compensated for by an increase in the weight percentage of protein. |
| topic |
chylomicrons very low density lipoproteins electron microscopy analytical centrifugation protein coverage |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520426501 |
| work_keys_str_mv |
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