An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA

An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA

International authorities classify the ricin toxin, present in castor seeds, as a potential agent for use in bioterrorism. Therefore, the detection, identification, and characterization of ricin are considered the first actions for its risk assessment during a suspected exposure, parallel to the dev...

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Bibliographic Details
Main Authors: George Oliveira, José Maurício Schneedorf
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Life
Subjects:
ricin
square wave voltammetry
kinetic analysis
hsDNA
Online Access:https://www.mdpi.com/2075-1729/11/5/405
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spelling doaj-03f310d15a724291b9aaa39fe8fb37bf2021-04-29T23:00:17ZengMDPI AGLife2075-17292021-04-011140540510.3390/life11050405An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNAGeorge Oliveira0José Maurício Schneedorf1Department of Biochemistry, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas 37130-000, BrazilDepartment of Biochemistry, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas 37130-000, BrazilInternational authorities classify the ricin toxin, present in castor seeds, as a potential agent for use in bioterrorism. Therefore, the detection, identification, and characterization of ricin are considered the first actions for its risk assessment during a suspected exposure, parallel to the development of therapeutic and medical countermeasures. In this study, we report the kinetic analysis of electro-oxidation of adenine released from hsDNA by the catalytic action of ricin by square wave voltammetry. The results suggest that ricin-mediated adenine release exhibited an unusual kinetic profile, with a progress curve controlled by the accumulation of the product and the values of the kinetic constants of 46.6 µM for <i>Km</i> and 2000 min<sup>−1</sup> for <i>kcat</i>, leading to a catalytic efficiency of 7.1 × 10<sup>5</sup> s<sup>−1</sup> M<sup>−1</sup>.https://www.mdpi.com/2075-1729/11/5/405ricinsquare wave voltammetrykinetic analysishsDNA
collection DOAJ
language English
format Article
sources DOAJ
author George Oliveira
José Maurício Schneedorf
spellingShingle George Oliveira
José Maurício Schneedorf
An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
Life
ricin
square wave voltammetry
kinetic analysis
hsDNA
author_facet George Oliveira
José Maurício Schneedorf
author_sort George Oliveira
title An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
title_short An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
title_full An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
title_fullStr An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
title_full_unstemmed An Electrochemical Approach to Follow and Evaluate the Kinetic Catalysis of Ricin on hsDNA
title_sort electrochemical approach to follow and evaluate the kinetic catalysis of ricin on hsdna
publisher MDPI AG
series Life
issn 2075-1729
publishDate 2021-04-01
description International authorities classify the ricin toxin, present in castor seeds, as a potential agent for use in bioterrorism. Therefore, the detection, identification, and characterization of ricin are considered the first actions for its risk assessment during a suspected exposure, parallel to the development of therapeutic and medical countermeasures. In this study, we report the kinetic analysis of electro-oxidation of adenine released from hsDNA by the catalytic action of ricin by square wave voltammetry. The results suggest that ricin-mediated adenine release exhibited an unusual kinetic profile, with a progress curve controlled by the accumulation of the product and the values of the kinetic constants of 46.6 µM for <i>Km</i> and 2000 min<sup>−1</sup> for <i>kcat</i>, leading to a catalytic efficiency of 7.1 × 10<sup>5</sup> s<sup>−1</sup> M<sup>−1</sup>.
topic ricin
square wave voltammetry
kinetic analysis
hsDNA
url https://www.mdpi.com/2075-1729/11/5/405
work_keys_str_mv AT georgeoliveira anelectrochemicalapproachtofollowandevaluatethekineticcatalysisofricinonhsdna
AT josemauricioschneedorf anelectrochemicalapproachtofollowandevaluatethekineticcatalysisofricinonhsdna
AT georgeoliveira electrochemicalapproachtofollowandevaluatethekineticcatalysisofricinonhsdna
AT josemauricioschneedorf electrochemicalapproachtofollowandevaluatethekineticcatalysisofricinonhsdna
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