Random amplified polymorphic DNA analysis of Anopheles nuneztovari (Diptera: Culicidae) from Western and Northeastern Colombia

• Main Authors: Carmen Elisa Posso, Ranulfo González, Heiber Cárdenas, Gerardo Gallego, Myriam Cristina Duque, Marco Fidel Suarez
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003-06-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Anopheles nuneztovari; genetic diversity; malaria vectors; Nyssorrhynchus; ramdon amplified polymorphic DNA; polymerase chain reaction; Colombia
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000400007
• ISSN: 0074-0276; 1678-8060

Random amplified polymorphic DNA (RAPD) markers were used to analyze 119 DNA samples of three Colombian Anopheles nuneztovari populations to study genetic variation and structure. Genetic diversity, estimated from heterozygosity, averaged 0.34. Genetic flow was greater between the two populations located in Western Colombia (F ST: 0.035; Nm: 6.8) but lower between these two and the northeastern population (F ST: 0.08; Nm: 2.8). According to molecular variance analysis, the genetic distance between populations was significant (phiST 0.1131, P < 0.001). The variation among individuals within populations (phiST 0.8869, P < 0.001)was also significant, suggesting a greater degree of population subdivision, not considered in this study. Both the parameters evaluated and the genetic flow suggest that Colombian An. nuneztovari populations are co-specific.