A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

Here, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The ds...

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Main Authors:	Jun Zi Zhu, Jun Guo, Zhao Hu, Xu Tong Zhang, Xiao Gang Li, Jie Zhong
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-06-01
Series:	Frontiers in Microbiology
Subjects:	partitivirus Colletotrichum liriopes hypovirulence dsRNA Colletotrichum liriopes partitivirus 1
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2021.653809/full

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spelling	doaj-03b82b2003994aa6ab50f1a1b6679f2b2021-06-23T16:38:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.653809653809A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopesJun Zi Zhu0Jun Zi Zhu1Jun Guo2Zhao Hu3Xu Tong Zhang4Xiao Gang Li5Xiao Gang Li6Jie Zhong7Hunan Engineering Research Center of Agricultural Pest Early Warning and Control, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHunan Engineering Research Center of Agricultural Pest Early Warning and Control, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha, ChinaHere, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The dsRNA 1 and dsRNA 2 encoded proteins showing significant amino acid (aa) sequence identity to the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of partitiviruses, respectively. Phylogenetic analysis using the aa sequences of RdRp and CP indicated that ClPV1 was grouped to members of the putative Epsilonpartitivirus genus in the Partitiviridae family. Spherical viral particles in approximately 35 nm in diameter and packaging the ClPV1 genome were isolated. Virus elimination and virus transfection with purified viral particles, and biological comparison revealed that ClPV1 could reduce the virulence and conidia production of C. liriopes. To the best of our knowledge, this is the first report of mycovirus in C. liriopes fungus.https://www.frontiersin.org/articles/10.3389/fmicb.2021.653809/fullpartitivirusColletotrichum liriopeshypovirulencedsRNAColletotrichum liriopes partitivirus 1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jun Zi Zhu Jun Zi Zhu Jun Guo Zhao Hu Xu Tong Zhang Xiao Gang Li Xiao Gang Li Jie Zhong
spellingShingle	Jun Zi Zhu Jun Zi Zhu Jun Guo Zhao Hu Xu Tong Zhang Xiao Gang Li Xiao Gang Li Jie Zhong A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes Frontiers in Microbiology partitivirus Colletotrichum liriopes hypovirulence dsRNA Colletotrichum liriopes partitivirus 1
author_facet	Jun Zi Zhu Jun Zi Zhu Jun Guo Zhao Hu Xu Tong Zhang Xiao Gang Li Xiao Gang Li Jie Zhong
author_sort	Jun Zi Zhu
title	A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes
title_short	A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes
title_full	A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes
title_fullStr	A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes
title_full_unstemmed	A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes
title_sort	novel partitivirus that confer hypovirulence to the plant pathogenic fungus colletotrichum liriopes
publisher	Frontiers Media S.A.
series	Frontiers in Microbiology
issn	1664-302X
publishDate	2021-06-01
description	Here, we report a novel double-stranded RNA virus designated Colletotrichum liriopes partitivirus 1 (ClPV1) from the plant pathogenic fungus C. liriopes. ClPV1 genome has two double stranded RNAs (dsRNAs), named as dsRNA 1 and dsRNA 2, which in the lengths of 1,807 and 1,706 bp, respectively. The dsRNA 1 and dsRNA 2 encoded proteins showing significant amino acid (aa) sequence identity to the RNA-dependent RNA polymerase (RdRp) and coat protein (CP) of partitiviruses, respectively. Phylogenetic analysis using the aa sequences of RdRp and CP indicated that ClPV1 was grouped to members of the putative Epsilonpartitivirus genus in the Partitiviridae family. Spherical viral particles in approximately 35 nm in diameter and packaging the ClPV1 genome were isolated. Virus elimination and virus transfection with purified viral particles, and biological comparison revealed that ClPV1 could reduce the virulence and conidia production of C. liriopes. To the best of our knowledge, this is the first report of mycovirus in C. liriopes fungus.
topic	partitivirus Colletotrichum liriopes hypovirulence dsRNA Colletotrichum liriopes partitivirus 1
url	https://www.frontiersin.org/articles/10.3389/fmicb.2021.653809/full
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A Novel Partitivirus That Confer Hypovirulence to the Plant Pathogenic Fungus Colletotrichum liriopes

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