| Summary: | The <i>let-7c</i> family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of <i>let-7c</i> in heart regeneration after injury in adult zebrafish. <i>let-7c</i> antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of <i>let-7c</i> increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker <i>raldh2</i> at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of <i>let-7c</i>. These results reveal a beneficial role of <i>let-7c</i> inhibition in adult zebrafish heart regeneration.
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