Introduction of Dianthins: A New Promising Horizon Toward Continuous Research on Breast Cancer Bulldozing in Iran

• Main Authors: Mohammad Hosseinpour, Jamil Zargan, Hossein Honari, Ashkan Haji Nour Mohammadi, Abbas Hajizadeh, Hani Keshavarz Alikhani, Ahmad Heidari, Ehsan Zamani
• Format: Article
• Language: English
• Published: Shaheed Beheshti University of Medical Sciences 2019-10-01
• Series: International Journal of Medical Toxicology and Forensic Medicine
• Subjects: dianthin; dianthus caryophyllus; mtt assay; neutral red uptake assay; mcf-7
• Online Access: https://journals.sbmu.ac.ir/ijmtfm/article/view/25706/pdf

Background: The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. The production and secretion of defense proteins are one of the protective mechanisms exploited by plants against pathogens. Ribosome-Inactivating Proteins (RIPs), as the main class of these proteins, are considered to facilitate cancer therapy worldwide, because of the potential anticancer activity. Indeed, some of these proteins have cytotoxic and anticancer properties. Extracted from the carnation (Dianthus caryophyllus), Dianthin inhibits protein synthesis in many different cells. Methods: In this research, the Dianthins was isolated and purified from the leaves of D. caryophyllus, using ion-exchange chromatography column (CM-Sephadex G-50). Subsequently, its cytotoxicity effect on MCF-7 cell line was investigated. The cell cytotoxicity assessment was performed, using 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT), neutral red uptake, and alkaline comet assays at the concentrations of 1.25μg/ mL to 10μg/mL of the protein applying the MCF-7 cell line. Results: the toxin induces cell death, mostly via necrosis rather than apoptosis, but in the special range of concentrations. Conclusion: because of the severe side effects of chemotherapy drugs, this toxin can undergo more research as a new drug candidate against breast cancer