Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR

In this study, an ultrasensitive detection method for aqueous Pb2+ based on digital polymerase chain reaction (dPCR) technology and a Pb2+-dependent DNAzyme was developed. In the presence of Pb2+, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting...

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Main Authors:	Tao Zhang, Cong Liu, Wuping Zhou, Keming Jiang, Chenyu Yin, Zhiqiang Zhang, Haiwen Li
Format:	Article
Language:	English
Published:	Hindawi Limited 2019-01-01
Series:	Journal of Analytical Methods in Chemistry
Online Access:	http://dx.doi.org/10.1155/2019/3528345

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record_format	Article
spelling	doaj-03949cf0ce384082acb60e3d655a0d692020-11-25T00:35:44ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/35283453528345Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCRTao Zhang0Cong Liu1Wuping Zhou2Keming Jiang3Chenyu Yin4Cong Liu5Zhiqiang Zhang6Haiwen Li7Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaKey Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaIn this study, an ultrasensitive detection method for aqueous Pb2+ based on digital polymerase chain reaction (dPCR) technology and a Pb2+-dependent DNAzyme was developed. In the presence of Pb2+, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in an increase in the amount of template DNA available for dPCR and a resultant change in the number of droplets showing a positive signal. Moreover, the detection system was found to be sensitive and stable in environmental sample detection. In summary, an ultrasensitive quantitative detection method for Pb2+ within environmental substrates was established.http://dx.doi.org/10.1155/2019/3528345
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Tao Zhang Cong Liu Wuping Zhou Keming Jiang Chenyu Yin Cong Liu Zhiqiang Zhang Haiwen Li
spellingShingle	Tao Zhang Cong Liu Wuping Zhou Keming Jiang Chenyu Yin Cong Liu Zhiqiang Zhang Haiwen Li Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR Journal of Analytical Methods in Chemistry
author_facet	Tao Zhang Cong Liu Wuping Zhou Keming Jiang Chenyu Yin Cong Liu Zhiqiang Zhang Haiwen Li
author_sort	Tao Zhang
title	Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
title_short	Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
title_full	Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
title_fullStr	Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
title_full_unstemmed	Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR
title_sort	ultrasensitive detection of pb2+ based on a dnazyme and digital pcr
publisher	Hindawi Limited
series	Journal of Analytical Methods in Chemistry
issn	2090-8865 2090-8873
publishDate	2019-01-01
description	In this study, an ultrasensitive detection method for aqueous Pb2+ based on digital polymerase chain reaction (dPCR) technology and a Pb2+-dependent DNAzyme was developed. In the presence of Pb2+, the Gr-5 DNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in an increase in the amount of template DNA available for dPCR and a resultant change in the number of droplets showing a positive signal. Moreover, the detection system was found to be sensitive and stable in environmental sample detection. In summary, an ultrasensitive quantitative detection method for Pb2+ within environmental substrates was established.
url	http://dx.doi.org/10.1155/2019/3528345
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Ultrasensitive Detection of Pb2+ Based on a DNAzyme and Digital PCR

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