Research on the promoting role of apelin-13 in proliferation, migration and capillary-like tube formation of RF/6A cells

• Main Authors: Kun-Peng Xie, Ping Liu, Xin Wang, Jun-Hui Du
• Format: Article
• Language: English
• Published: Press of International Journal of Ophthalmology (IJO PRESS) 2017-05-01
• Series: Guoji Yanke Zazhi
• Subjects: apelin; apelin-13; angiogenesis; retinal neovascularization
• Online Access: http://ies.ijo.cn/cn_publish/2017/6/201706008.pdf

AIM: To investigate the effects of apelin-13 on proliferation, migration and capillary-like tube formation of a monkey choroid / retinal endothelial cell line, RF/6A, to clarify whether apelin-13 could promote retinal angiogenesis <i>in vitro</i>.<p>METHODS: RF/6A cells in good conditions were administrated with DMSO(the control group), apelin-13 at 0.1μmol/L(low dose group)or apelin-13 at 1μmol/L(high dose group). Cell proliferation, migration and capillary-like tube formation were detected by using the MTT assay, scratch assay and matrigel assay, respectively, at 24h after plating the cells. <p>RESULTS: Cell proliferation was promoted in both low and high dose apelin-13 groups compared to the control cells(<i>P</i><0.05); the cell migration distance of both apelin-13 groups was significantly greater than that of the control group(<i>P</i><0.05); and the number of capillary-like tube structures of both apelin-13 groups was significantly larger than that of the control cells(<i>P</i><0.05). In addition, cell proliferation, migration and tube formation increased as the concentration of apelin-13 increased. <p>CONCLUSION: Apelin-13 could obviously promote the angiogenesis capacity of RF/6A cells, suggesting that apelin-13 was an important pro-angiogenic factor in retinal endothelial cells.