A method for generation of bone marrow-derived macrophages from cryopreserved mouse bone marrow cells.

The broad use of transgenic and gene-targeted mice has established bone marrow-derived macrophages (BMDM) as important mammalian host cells for investigation of the macrophages biology. Over the last decade, extensive research has been done to determine how to freeze and store viable hematopoietic h...

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Bibliographic Details
Main Authors: Fernanda M Marim, Tatiana N Silveira, Djalma S Lima, Dario S Zamboni
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3003694?pdf=render