Evaluation of anthocyanins in Aronia melanocarpa/BSA binding by spectroscopic studies

• Main Authors: Jie Wei, Dexin Xu, Xiao Zhang, Jing Yang, Qiuyu Wang
• Format: Article
• Language: English
• Published: SpringerOpen 2018-05-01
• Series: AMB Express
• Subjects: Anthocyanins in Aronia melanocarpa; BSA; Binding mode; Circular dichroism; Molecular docking
• Online Access: http://link.springer.com/article/10.1186/s13568-018-0604-5

Abstract The interaction between Anthocyanins in Aronia melanocarpa (AMA) and bovine serum albumin (BSA) were studied in this paper by multispectral technology, such as fluorescence quenching titration, circular dichroism (CD) spectroscopy and Fourier transform infrared spectroscopy (FTIR). The results of the fluorescence titration revealed that AMA could strongly quench the intrinsic fluorescence of BSA by static quenching. The apparent binding constants KSV and number of binding sites n of AMA with BSA were obtained by fluorescence quenching method. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), were calculated to be 18.45 kJ mol−1 > 0 and 149.72 J mol−1 K−1 > 0, respectively, which indicated that the interaction of AMA with BSA was driven mainly by hydrophobic forces. The binding process was a spontaneous process of Gibbs free energy change. Based on Förster’s non-radiative energy transfer theory, the distance r between the donor (BSA) and the receptor (AMA) was calculated to be 3.88 nm. Their conformations were analyzed using infrared spectroscopy and CD. The results of multispectral technology showed that the binding of AMA to BSA induced the conformational change of BSA.