Inhibition effects of dexamethasone on matrix metalloproteinase in osteoarthritis cells

• Main Authors: Guang-Yao Chen, Qing-Wen Tao
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2021-05-01
• Series: Journal of Hainan Medical University
• Subjects: osteoarthritis; dexamethasone; sw1353 cells; interleukin -1β
• Online Access: http://www.hnykdxxb.com/PDF/202110/07.pdf

Objective: To investigate the inhibition effect of dexamethasone with different concentration gradients on the expression of MMP-1, -3, and -13 in a bone joint cell model of IL-1β-induced SW1353 cells. Methods: The SW1353 cells were placed in a culture medium containing 101-108 nmol/L dexamethasone, and after 12 hours of culture, the appropriate intervention concentration range of dexamethasone was screened by the MTS for subsequent experiments. After SW1353 cells were induced with 10 ng/mL IL-1β, 101-107 nmol/L dexamethasone was given for intervention, and RT-PCR was used to detect the mRNA expression of MMP-1, -3, and -13. The protein was extracted after the intervention of 104 nmol/L dexamethasone, and western blot was used to detect the protein expression of MMP-1, -3, and -13. Results: MTS proliferation experiment results showed that 101-107 nmol/L dexamethasone had no significant effect on SW1353 cell viability, while 108 nmol/L dexamethasone had a significant inhibitory effect on SW1353 (p <0.05). There was no obvious effect on cell viability after adding 10 ng/mL IL-1β. RT-PCR results indicated that the IL-1β group had a significant increase in the mRNA expression of MMP-1, -3, and -13 compared with the blank control group, and the difference was statistically significant (p <0.05). After intervention with 102-107 nmol/L dexamethasone, MMP-1 mRNA expression decreased (p <0.05); after intervention with 103- 107 nmol/L dexamethasone, MMP-3 mRNA expression decreased (p < 0.05); after intervention with 10-107 nmol/L dexamethasone, the mRNA expression of MMP-13 decreased (p <0.05). The Western blot results showed that compared with the blank control group, the protein levels of MMP-1, -3, and -13 in the IL-1β group were significantly increased; the protein levels of MMP-1, -3, and -13 were significantly reduced after the intervention of 104 nmol/L dexamethasone. Conclusion: Dexamethasone can inhibit the expression of MMP1, MMP-3, and MMP-13 in the IL-1β-induced OA cell model at the mRNA and protein levels.