Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway

Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway

The mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that in non-CRPC cells. Silencing CAPN2 expression could inhibit DU145 and PC3 cells proliferation by cell cycle arrest at G1 phase. Knockdown of CPAN2 level suppressed the migration and invasion capacity of CRPC cells b...

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Main Authors: Pu Li, Chenkui Miao, Chao Liang, Pengfei Shao, Zengjun Wang, Jie Li
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2017/2593674
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spelling doaj-031f2ca60c1a4fe098afe2b4e49fb0d32020-11-24T22:01:53ZengHindawi LimitedBioMed Research International2314-61332314-61412017-01-01201710.1155/2017/25936742593674Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal PathwayPu Li0Chenkui Miao1Chao Liang2Pengfei Shao3Zengjun Wang4Jie Li5State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaState Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, ChinaThe mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that in non-CRPC cells. Silencing CAPN2 expression could inhibit DU145 and PC3 cells proliferation by cell cycle arrest at G1 phase. Knockdown of CPAN2 level suppressed the migration and invasion capacity of CRPC cells by reducing matrix metalloproteinase-2 (MMP-2) and MMP-9 activation, as well as repressing the phosphorylation protein expression of AKT and mTOR. In addition, we found that the expression of CAPN2 was elevated in Pca tissues than that in normal control tissues. Therefore, we showed the important roles of CAPN2 in the development and progression in CRPC cells, suggesting a new therapeutic intervention for treating castration-resistant prostate cancer patients.http://dx.doi.org/10.1155/2017/2593674
collection DOAJ
language English
format Article
sources DOAJ
author Pu Li
Chenkui Miao
Chao Liang
Pengfei Shao
Zengjun Wang
Jie Li
spellingShingle Pu Li
Chenkui Miao
Chao Liang
Pengfei Shao
Zengjun Wang
Jie Li
Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
BioMed Research International
author_facet Pu Li
Chenkui Miao
Chao Liang
Pengfei Shao
Zengjun Wang
Jie Li
author_sort Pu Li
title Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
title_short Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
title_full Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
title_fullStr Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
title_full_unstemmed Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway
title_sort silencing capn2 expression inhibited castration-resistant prostate cancer cells proliferation and invasion via akt/mtor signal pathway
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2017-01-01
description The mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that in non-CRPC cells. Silencing CAPN2 expression could inhibit DU145 and PC3 cells proliferation by cell cycle arrest at G1 phase. Knockdown of CPAN2 level suppressed the migration and invasion capacity of CRPC cells by reducing matrix metalloproteinase-2 (MMP-2) and MMP-9 activation, as well as repressing the phosphorylation protein expression of AKT and mTOR. In addition, we found that the expression of CAPN2 was elevated in Pca tissues than that in normal control tissues. Therefore, we showed the important roles of CAPN2 in the development and progression in CRPC cells, suggesting a new therapeutic intervention for treating castration-resistant prostate cancer patients.
url http://dx.doi.org/10.1155/2017/2593674
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AT chenkuimiao silencingcapn2expressioninhibitedcastrationresistantprostatecancercellsproliferationandinvasionviaaktmtorsignalpathway
AT chaoliang silencingcapn2expressioninhibitedcastrationresistantprostatecancercellsproliferationandinvasionviaaktmtorsignalpathway
AT pengfeishao silencingcapn2expressioninhibitedcastrationresistantprostatecancercellsproliferationandinvasionviaaktmtorsignalpathway
AT zengjunwang silencingcapn2expressioninhibitedcastrationresistantprostatecancercellsproliferationandinvasionviaaktmtorsignalpathway
AT jieli silencingcapn2expressioninhibitedcastrationresistantprostatecancercellsproliferationandinvasionviaaktmtorsignalpathway
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