Effect of Monophosphoryl Lipid A on Lipopolysaccharide-Induced Nitric Oxide Production from Raw 264.7 Macrophages

• Main Authors: Cemalettin AYBAY, Turgut İMİR, H. Cengiz GÜLSAYIN, Başak KAYHAN
• Format: Article
• Language: English
• Published: Bilimsel Tip Yayinevi 1997-06-01
• Series: Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi
• Subjects: Lypopolysaccharide (LPS); Monophosphoryl lipid A; Nitric Oxide (NO)
• Online Access: http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=1997-2-2-120-126.pdf

The lipid A moiety of LPS is responsible for the toxic effects of LPS. The identification of structural analogs and precursors of lipid A have demonstrated that they are apparently competitive antagonists against the biological actions of LPS. In this study we investigated the effect of Salmonella Minnesota monophosphoryl lipid A on nitric oxide (NO) secretion from Salmonella minnesota LPS-stimulated RAW 264.7 macrophages. Although monophosphoryl lipid A itself induced NO production from macrophages in a dose dependent manner, at a given concentration, monophosphoryl lipid A demonstrated inhibitory action on LPS-induced NO production from macrophages. At the concentrations equal to and lower than 0.125 µg/mL, NO inducing capacity of monophosphoryl lipid A significantly decreased along with reducing the LPS-induced NO production from RAW264.7 macrophages.