Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer
Extracellular vesicles (EVs) are involved in intercellular communication during the carcinogenesis. Our attention has been focused on small EVs (sEVs) protein content in colorectal and gastric cancer (CRC and GC). Frizzled (FZD) proteins, a family of receptors comprised in the Wnt signaling pathway,...
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Series: | Journal of Oncology |
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doaj-02fb72ffbd6d4f7abcfe41030bb9ee672020-11-24T21:21:29ZengHindawi LimitedJournal of Oncology1687-84501687-84692019-01-01201910.1155/2019/27159682715968Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric CancerScavo Maria Principia0Cigliano Antonio1Depalo Nicoletta2Fanizza Elisabetta3Maria Grazia Bianco4Denora Nunzio5Laquintana Valentino6Curri Maria Lucia7Lorusso Dionigi8Lotesoriere Claudio9Panarese Alba10Giannelli Gianluigi11Personalized Medicine Laboratory, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyPersonalized Medicine Laboratory, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyInstitute for Chemical-Physical Processes (IPCF)-CNR, SS Bari, Via Orabona 4, 70125 Bari, ItalyInstitute for Chemical-Physical Processes (IPCF)-CNR, SS Bari, Via Orabona 4, 70125 Bari, ItalyPersonalized Medicine Laboratory, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyInstitute for Chemical-Physical Processes (IPCF)-CNR, SS Bari, Via Orabona 4, 70125 Bari, ItalyUniversità degli Studi di Bari Aldo Moro, Dipartimento di Farmacia, Scienze del Farmaco, Via Orabona 4, 70125 Bari, ItalyInstitute for Chemical-Physical Processes (IPCF)-CNR, SS Bari, Via Orabona 4, 70125 Bari, ItalySurgical Gastroenterology Unit, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyOncology Unit, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyEndoscopy Unit, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyPersonalized Medicine Laboratory, National Institute of Gastroenterology “S. De Bellis” Research Hospital, Via Turi 27, Castellana Grotte, Bari, ItalyExtracellular vesicles (EVs) are involved in intercellular communication during the carcinogenesis. Our attention has been focused on small EVs (sEVs) protein content in colorectal and gastric cancer (CRC and GC). Frizzled (FZD) proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in the carcinogenesis of CRC and GC. Here, the expression of a specific FZD protein, namely, FZD-10, was investigated in the sEVs extracted from plasma of patients affected by CRC and GC as involved in canonical and noncanonical Wnt signaling in cancer stem cells with a subsequent modification of cellular heterogeneity, omics reprogramming, and tumor plasticity. The expression of FZD-10 protein in the sEVs extracted from plasma of patients affected by CRC and GC and sEVs from plasma of healthy subjects was evaluated against the level of protein Hsp70, established as EVs specific markers along with CD63 and ALIX proteins. The FZD-10 extract from sEVs isolated from plasma of the controls and the CRC or GC subjects indicated that its expression in oncological patients was higher than in the control group, while, at the end of the treatment, it reached values comparable with the average level of controls. Furthermore, the level of FZD-10 in the whole plasma was found comparable with its level in the sEVs extract. The level of FZD-10 in the sEVs represents a potential reliable biomarker with a valuable prognostic function for the diagnosis of CRC and GC and for monitoring the treatment response.http://dx.doi.org/10.1155/2019/2715968 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Scavo Maria Principia Cigliano Antonio Depalo Nicoletta Fanizza Elisabetta Maria Grazia Bianco Denora Nunzio Laquintana Valentino Curri Maria Lucia Lorusso Dionigi Lotesoriere Claudio Panarese Alba Giannelli Gianluigi |
spellingShingle |
Scavo Maria Principia Cigliano Antonio Depalo Nicoletta Fanizza Elisabetta Maria Grazia Bianco Denora Nunzio Laquintana Valentino Curri Maria Lucia Lorusso Dionigi Lotesoriere Claudio Panarese Alba Giannelli Gianluigi Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer Journal of Oncology |
author_facet |
Scavo Maria Principia Cigliano Antonio Depalo Nicoletta Fanizza Elisabetta Maria Grazia Bianco Denora Nunzio Laquintana Valentino Curri Maria Lucia Lorusso Dionigi Lotesoriere Claudio Panarese Alba Giannelli Gianluigi |
author_sort |
Scavo Maria Principia |
title |
Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer |
title_short |
Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer |
title_full |
Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer |
title_fullStr |
Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer |
title_full_unstemmed |
Frizzled-10 Extracellular Vesicles Plasma Concentration Is Associated with Tumoral Progression in Patients with Colorectal and Gastric Cancer |
title_sort |
frizzled-10 extracellular vesicles plasma concentration is associated with tumoral progression in patients with colorectal and gastric cancer |
publisher |
Hindawi Limited |
series |
Journal of Oncology |
issn |
1687-8450 1687-8469 |
publishDate |
2019-01-01 |
description |
Extracellular vesicles (EVs) are involved in intercellular communication during the carcinogenesis. Our attention has been focused on small EVs (sEVs) protein content in colorectal and gastric cancer (CRC and GC). Frizzled (FZD) proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in the carcinogenesis of CRC and GC. Here, the expression of a specific FZD protein, namely, FZD-10, was investigated in the sEVs extracted from plasma of patients affected by CRC and GC as involved in canonical and noncanonical Wnt signaling in cancer stem cells with a subsequent modification of cellular heterogeneity, omics reprogramming, and tumor plasticity. The expression of FZD-10 protein in the sEVs extracted from plasma of patients affected by CRC and GC and sEVs from plasma of healthy subjects was evaluated against the level of protein Hsp70, established as EVs specific markers along with CD63 and ALIX proteins. The FZD-10 extract from sEVs isolated from plasma of the controls and the CRC or GC subjects indicated that its expression in oncological patients was higher than in the control group, while, at the end of the treatment, it reached values comparable with the average level of controls. Furthermore, the level of FZD-10 in the whole plasma was found comparable with its level in the sEVs extract. The level of FZD-10 in the sEVs represents a potential reliable biomarker with a valuable prognostic function for the diagnosis of CRC and GC and for monitoring the treatment response. |
url |
http://dx.doi.org/10.1155/2019/2715968 |
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