A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes

A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes

Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcription...

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Main Authors: Sheila S Teves, Luye An, Aarohi Bhargava-Shah, Liangqi Xie, Xavier Darzacq, Robert Tjian
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2018-06-01
Series:eLife
Subjects:
mouse embryonic stem cells
transcription factors
TBP
single molecule live-cell imaging
Online Access:https://elifesciences.org/articles/35621
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spelling doaj-02ea2452818b46efb62a0861fa8552c32021-05-05T15:58:32ZengeLife Sciences Publications LtdeLife2050-084X2018-06-01710.7554/eLife.35621A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomesSheila S Teves0https://orcid.org/0000-0002-1220-2414Luye An1Aarohi Bhargava-Shah2Liangqi Xie3Xavier Darzacq4https://orcid.org/0000-0003-2537-8395Robert Tjian5https://orcid.org/0000-0003-0539-8217Department of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesDepartment of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, United StatesDepartment of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, Li Ka Shing Center for Biomedical and Health Sciences, CIRM Center of Excellence, University of California, Berkeley, Berkeley, United States; Howard Hughes Medical Institute, Berkeley, United StatesMaintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.https://elifesciences.org/articles/35621mouse embryonic stem cellstranscription factorsTBPsingle molecule live-cell imaging
collection DOAJ
language English
format Article
sources DOAJ
author Sheila S Teves
Luye An
Aarohi Bhargava-Shah
Liangqi Xie
Xavier Darzacq
Robert Tjian
spellingShingle Sheila S Teves
Luye An
Aarohi Bhargava-Shah
Liangqi Xie
Xavier Darzacq
Robert Tjian
A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
eLife
mouse embryonic stem cells
transcription factors
TBP
single molecule live-cell imaging
author_facet Sheila S Teves
Luye An
Aarohi Bhargava-Shah
Liangqi Xie
Xavier Darzacq
Robert Tjian
author_sort Sheila S Teves
title A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
title_short A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
title_full A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
title_fullStr A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
title_full_unstemmed A stable mode of bookmarking by TBP recruits RNA polymerase II to mitotic chromosomes
title_sort stable mode of bookmarking by tbp recruits rna polymerase ii to mitotic chromosomes
publisher eLife Sciences Publications Ltd
series eLife
issn 2050-084X
publishDate 2018-06-01
description Maintenance of transcription programs is challenged during mitosis when chromatin becomes condensed and transcription is silenced. How do the daughter cells re-establish the original transcription program? Here, we report that the TATA-binding protein (TBP), a key component of the core transcriptional machinery, remains bound globally to active promoters in mouse embryonic stem cells during mitosis. Using live-cell single-molecule imaging, we observed that TBP mitotic binding is highly stable, with an average residence time of minutes, in stark contrast to typical TFs with residence times of seconds. To test the functional effect of mitotic TBP binding, we used a drug-inducible degron system and found that TBP promotes the association of RNA Polymerase II with mitotic chromosomes, and facilitates transcriptional reactivation following mitosis. These results suggest that the core transcriptional machinery promotes efficient transcription maintenance globally.
topic mouse embryonic stem cells
transcription factors
TBP
single molecule live-cell imaging
url https://elifesciences.org/articles/35621
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