Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue

Morbidity from calcific aortic valve disease (CAVD) is increasing. Recent studies suggest early reversible changes involving inflammation and neoangiogenesis. We hypothesized that microcalcifications, chemokines, and growth factors are present in unaffected regions of calcific aortic valves. We stu...

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Main Authors: P. Dorfmüller, D. Bazin, S. Aubert, R. Weil, F. Brisset, M. Daudon, F. Capron, I. Brochériou
Format: Article
Language:English
Published: Hindawi Limited 2010-01-01
Series:Cardiology Research and Practice
Online Access:http://dx.doi.org/10.4061/2010/685926
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spelling doaj-02e4c0d4ba294df3b25118a9be04109c2020-11-24T22:36:30ZengHindawi LimitedCardiology Research and Practice2090-05972010-01-01201010.4061/2010/685926685926Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve TissueP. Dorfmüller0D. Bazin1S. Aubert2R. Weil3F. Brisset4M. Daudon5F. Capron6I. Brochériou7Service d'Anatomie et de Cytologie Pathologiques, Hôpital de la Pitié-Salpêtrière, 47-80 Boulevard de l'Hôpital, Assistance Publique-Hôpitaux de Paris, Université Pierre et Marie Curie, 75013 Paris, FranceLaboratoire de Physique des Solides, UMR 8502, Université Paris-Sud Orsay, Bâtiment 510, 91405 Orsay, FranceService de Chirurgie Cardiaque, Hôpital de la Pitié-Salpêtrière, 47-80 Boulevard de l'Hôpital, Assistance Publique-Hôpitaux de Paris, Université Pierre et Marie Curie, 75013 Paris, FranceLaboratoire de Physique des Solides, UMR 8502, Université Paris-Sud Orsay, Bâtiment 510, 91405 Orsay, FranceLaboratoire de Physique des Solides, UMR 8502, Université Paris-Sud Orsay, Bâtiment 510, 91405 Orsay, FranceLaboratoire de Physique des Solides, UMR 8502, Université Paris-Sud Orsay, Bâtiment 510, 91405 Orsay, FranceService d'Anatomie et de Cytologie Pathologiques, Hôpital de la Pitié-Salpêtrière, 47-80 Boulevard de l'Hôpital, Assistance Publique-Hôpitaux de Paris, Université Pierre et Marie Curie, 75013 Paris, FranceService d'Anatomie et de Cytologie Pathologiques, Hôpital de la Pitié-Salpêtrière, 47-80 Boulevard de l'Hôpital, Assistance Publique-Hôpitaux de Paris, Université Pierre et Marie Curie, 75013 Paris, FranceMorbidity from calcific aortic valve disease (CAVD) is increasing. Recent studies suggest early reversible changes involving inflammation and neoangiogenesis. We hypothesized that microcalcifications, chemokines, and growth factors are present in unaffected regions of calcific aortic valves. We studied aortic valves from 4 patients with CAVD and from 1 control, using immunohistochemistry, scanning electron microscopy, and infrared spectrography. We revealed clusters of capillary neovessels in calcified (ECC), to a lesser extent in noncalcified (ECN) areas. Endothelial cells proved constant expression of SDF-1 in ECC, ECN, and endothelial cells from valvular surface (ECS). Its receptor CXCR4 was expressed in ECC. IL-6 expression correlated with CXCR4 staining and presence of lymphocytes. VEGF was expressed by ECS, its receptor by ECC and ECN. Crystalline ultrastructures were found on the surface of histologically noncalcified areas (HNCAs), spectrography revealed calcium hydroxylapatite. Our results demonstrate that crystalline ultrastructures are present in HNCAs, undergoing neoangiogenesis in an inflammatory context. These alterations could be an early witness of disease and an opening to therapy.http://dx.doi.org/10.4061/2010/685926
collection DOAJ
language English
format Article
sources DOAJ
author P. Dorfmüller
D. Bazin
S. Aubert
R. Weil
F. Brisset
M. Daudon
F. Capron
I. Brochériou
spellingShingle P. Dorfmüller
D. Bazin
S. Aubert
R. Weil
F. Brisset
M. Daudon
F. Capron
I. Brochériou
Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
Cardiology Research and Practice
author_facet P. Dorfmüller
D. Bazin
S. Aubert
R. Weil
F. Brisset
M. Daudon
F. Capron
I. Brochériou
author_sort P. Dorfmüller
title Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
title_short Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
title_full Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
title_fullStr Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
title_full_unstemmed Crystalline Ultrastructures, Inflammatory Elements, and Neoangiogenesis Are Present in Inconspicuous Aortic Valve Tissue
title_sort crystalline ultrastructures, inflammatory elements, and neoangiogenesis are present in inconspicuous aortic valve tissue
publisher Hindawi Limited
series Cardiology Research and Practice
issn 2090-0597
publishDate 2010-01-01
description Morbidity from calcific aortic valve disease (CAVD) is increasing. Recent studies suggest early reversible changes involving inflammation and neoangiogenesis. We hypothesized that microcalcifications, chemokines, and growth factors are present in unaffected regions of calcific aortic valves. We studied aortic valves from 4 patients with CAVD and from 1 control, using immunohistochemistry, scanning electron microscopy, and infrared spectrography. We revealed clusters of capillary neovessels in calcified (ECC), to a lesser extent in noncalcified (ECN) areas. Endothelial cells proved constant expression of SDF-1 in ECC, ECN, and endothelial cells from valvular surface (ECS). Its receptor CXCR4 was expressed in ECC. IL-6 expression correlated with CXCR4 staining and presence of lymphocytes. VEGF was expressed by ECS, its receptor by ECC and ECN. Crystalline ultrastructures were found on the surface of histologically noncalcified areas (HNCAs), spectrography revealed calcium hydroxylapatite. Our results demonstrate that crystalline ultrastructures are present in HNCAs, undergoing neoangiogenesis in an inflammatory context. These alterations could be an early witness of disease and an opening to therapy.
url http://dx.doi.org/10.4061/2010/685926
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