Variation of autosomes and X chromosome STR in breast cancer and gynecological cancer tissues

• Main Authors: Hou Youxiang, Dong Zhanfei, Kuerban Gulina
• Format: Article
• Language: English
• Published: De Gruyter 2017-04-01
• Series: Open Life Sciences
• Subjects: gynecological cancer; breast cancer; autosomes; x chromosome; str variation typing
• Online Access: https://doi.org/10.1515/biol-2017-0012

This study analyses 1000 cases of patients with breast cancer and 2000 cases of patients with gynecological cancer (1000 cases of malignant tumor, 1000 cases of benign tumors), where breast cancer and malignant tumor patients comprise the observation group, while patients with benign tumors comprise the control group. Through DNA extraction, STR genotyping and variation verification, microdissection, individual STR mutation rate and loci STR mutation rate of the two groups of patients were calculated. Results show that there are no significant (P > 0.05) differences in the STR variation of autosomes and X chromosome between patients in the observation group and those in the reference group. However, significant (P < 0.05) intergroup differences were found for STR variation typing between patients with malignant and benign tumors. Using STR genotyping for autosomes and X chromosomes, gynecological cancer patients were found to be more likely to mutate, with a clear relationship between STR variation and tumor differentiation degrees. The study on the variation analysis of autosomes and X chromosome STR in breast and gynecological cancer tissues is expected to have a high application value when applied to medical research and identification processes.