Quantification of Phenolic Constituents and Antioxidant Activity of Pterodon emarginatus Vogel Seeds

• Main Authors: Nádia R. Barbosa, Magda N. Leite, Rafael C. Dutra
• Format: Article
• Language: English
• Published: MDPI AG 2008-04-01
• Series: International Journal of Molecular Sciences
• Subjects: Pterodon emarginatus; Leguminosae; phenolic constituents; DPPH.
• Online Access: http://www.mdpi.com/1422-0067/9/4/606/

In the present study the phenolic (Folin-Dennis) and flavonoid (colorimetric assay) constituents and the antioxidant activity of Pterodon emarginatus seeds were investigated in several samples prepared with different extraction procedures: essential oil (EO) using a Clevenger-type apparatus; hexanic (HF), ethyl acetate (EAF), buthanolic (BF) and methanolic (MF) fractions using Soxhlet extraction, and extracts (1 g/extract) obtained from different methods: reflux 80°C/30 min, ultrasound/30 min, static maceration/48 h and heating plate 100°C/45 min. These extracts were prepared using water or ethanol/water at 30:70 v/v, 50:50 v/v or 70:30 v/v. Antioxidant activity [2,2-diphenyl-2- picrylhydrazyl hydrate (DPPH)] was tested only in the fractions obtained from Soxhlet extraction. The extract obtained from reflux using ethanol/water (70:30, v/v) showed the highest phenolic constituents level. The EAF, BF and MF showed DPPH scavenging activities with IC50=163.22, 18.89 and 10.15 μg/ml, respectively.