Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor

A label-free biosensor based on silicon-on-insulator (SOI) photonic bandgap (PBG) structures is performed for specific protein detection. First, the SOI sensing surface is functionalized using triethoxyvinylsilane (TEVS) organosilane. Then, a UV light photocatalyzed immobilization of polyclonal half...

Full description

Bibliographic Details
Main Authors:	Jad Sabek, Luis Torrijos-Morán, Zeneida Díaz-Betancor, María José Bañuls-Polo, Ángel Maquieira-Catalá, Jaime García-Rupérez
Format:	Article
Language:	English
Published:	MDPI AG 2019-01-01
Series:	Proceedings
Subjects:	photonic sensor photonic bandgap silicon on insulator biofunctionalization photocatalysis half antibodies
Online Access:	https://www.mdpi.com/2504-3900/4/1/15

id	doaj-02bb8b5423f0430aab6390d558d53529
record_format	Article
spelling	doaj-02bb8b5423f0430aab6390d558d535292020-11-24T21:34:37ZengMDPI AGProceedings2504-39002019-01-01411510.3390/ecsa-5-05718ecsa-5-05718Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic BiosensorJad Sabek0Luis Torrijos-Morán1Zeneida Díaz-Betancor2María José Bañuls-Polo3Ángel Maquieira-Catalá4Jaime García-Rupérez5Nanophotonics Technology Center, Universitat Politècnica de València. Camí de Vera s/n, Valencia, SpainNanophotonics Technology Center, Universitat Politècnica de València. Camí de Vera s/n, Valencia, SpainDepartamento de Química, Instituto Interuniversitario de Investigación y de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, Valencia, SpainDepartamento de Química, Instituto Interuniversitario de Investigación y de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, Valencia, SpainDepartamento de Química, Instituto Interuniversitario de Investigación y de Reconocimiento Molecular y Desarrollo Tecnológico IDM, Universitat Politècnica de València, Camí de Vera s/n, Valencia, SpainNanophotonics Technology Center, Universitat Politècnica de València. Camí de Vera s/n, Valencia, SpainA label-free biosensor based on silicon-on-insulator (SOI) photonic bandgap (PBG) structures is performed for specific protein detection. First, the SOI sensing surface is functionalized using triethoxyvinylsilane (TEVS) organosilane. Then, a UV light photocatalyzed immobilization of polyclonal half anti-bovine serum albumin (haBSA) antibodies is performed. Finally, a direct detection of target BSA antigen is carried out. Both the immobilization and the detection steps are monitored by making a continuous tracking of the PBG edge shift. In order to confirm the recognition of the antigen by the immobilized antibody, a fluorophore-labelled secondary antibody was flowed at the end of the experiment in order to perform a confirmation fluorescence test after the photonic detection.https://www.mdpi.com/2504-3900/4/1/15photonic sensorphotonic bandgapsilicon on insulatorbiofunctionalizationphotocatalysishalf antibodies
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jad Sabek Luis Torrijos-Morán Zeneida Díaz-Betancor María José Bañuls-Polo Ángel Maquieira-Catalá Jaime García-Rupérez
spellingShingle	Jad Sabek Luis Torrijos-Morán Zeneida Díaz-Betancor María José Bañuls-Polo Ángel Maquieira-Catalá Jaime García-Rupérez Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor Proceedings photonic sensor photonic bandgap silicon on insulator biofunctionalization photocatalysis half antibodies
author_facet	Jad Sabek Luis Torrijos-Morán Zeneida Díaz-Betancor María José Bañuls-Polo Ángel Maquieira-Catalá Jaime García-Rupérez
author_sort	Jad Sabek
title	Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor
title_short	Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor
title_full	Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor
title_fullStr	Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor
title_full_unstemmed	Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor
title_sort	live tracking biofunctionalization and label-free protein detection performed by a nanophotonic biosensor
publisher	MDPI AG
series	Proceedings
issn	2504-3900
publishDate	2019-01-01
description	A label-free biosensor based on silicon-on-insulator (SOI) photonic bandgap (PBG) structures is performed for specific protein detection. First, the SOI sensing surface is functionalized using triethoxyvinylsilane (TEVS) organosilane. Then, a UV light photocatalyzed immobilization of polyclonal half anti-bovine serum albumin (haBSA) antibodies is performed. Finally, a direct detection of target BSA antigen is carried out. Both the immobilization and the detection steps are monitored by making a continuous tracking of the PBG edge shift. In order to confirm the recognition of the antigen by the immobilized antibody, a fluorophore-labelled secondary antibody was flowed at the end of the experiment in order to perform a confirmation fluorescence test after the photonic detection.
topic	photonic sensor photonic bandgap silicon on insulator biofunctionalization photocatalysis half antibodies
url	https://www.mdpi.com/2504-3900/4/1/15
work_keys_str_mv	AT jadsabek livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor AT luistorrijosmoran livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor AT zeneidadiazbetancor livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor AT mariajosebanulspolo livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor AT angelmaquieiracatala livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor AT jaimegarciaruperez livetrackingbiofunctionalizationandlabelfreeproteindetectionperformedbyananophotonicbiosensor
_version_	1725948599693475840

Live Tracking Biofunctionalization and Label-Free Protein Detection Performed by a Nanophotonic Biosensor

Similar Items