Development and Initial Characterization of a HAPPY Panel for Mapping the X. Tropicalis Genome

• Main Author: Zhihua Jiang, Jennifer J. Michal, Kenneth B. Beckman, Jessica B. Lyons, Ming Zhang, Zengxiang Pan, Daniel S. Rokhsar, Richard M. Harland
• Format: Article
• Language: English
• Published: Ivyspring International Publisher 2011-01-01
• Series: International Journal of Biological Sciences
• Online Access: http://www.biolsci.org/v07p1037.htm

<p>HAPPY mapping was designed to pursue the analysis of approximately random <u>HAP</u>loid DNA breakage samples using the <u>P</u>ol<u>Y</u>merase chain reaction for mapping genomes. In the present study, we improved the method and integrated two other molecular techniques into the process: whole genome amplification and the Sequenom SNP (single nucleotide polymorphism) genotyping assay in order to facilitate whole genome mapping of <i>X. tropicalis</i>. The former technique amplified enough DNA materials to genotype a large number of markers, while the latter allowed for relatively high throughput marker genotyping with multiplex assays on the HAPPY lines. A total of 58 <i>X. tropicalis</i> genes were genotyped on an initial panel of 383 HAPPY lines, which contributed to formation of a working panel of 146 lines. Further genotyping of 29 markers on the working panel led to construction of a HAPPY map for the <i>X. tropicalis</i> genome. We believe that our improved HAPPY method described in the present study has paved the way for the community to map different genomes with a simple, but powerful approach.</p>