StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.

Infection with Streptococcus pyogenes (S. pyogenes) can result in several diseases, particularly in children. S. pyogenes M protein is the major virulence factor, and certain regions of its N-terminus can trigger autoimmune sequelae such as rheumatic fever in susceptible individuals with untreated g...

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Main Authors: Edilberto Postol, Raquel Alencar, Fabio T Higa, Samar Freschi de Barros, Lea M F Demarchi, Jorge Kalil, Luiza Guilherme
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3620221?pdf=render
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spelling doaj-0235416479ea4224bc8102df7085ffaf2020-11-25T01:19:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6096910.1371/journal.pone.0060969StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.Edilberto PostolRaquel AlencarFabio T HigaSamar Freschi de BarrosLea M F DemarchiJorge KalilLuiza GuilhermeInfection with Streptococcus pyogenes (S. pyogenes) can result in several diseases, particularly in children. S. pyogenes M protein is the major virulence factor, and certain regions of its N-terminus can trigger autoimmune sequelae such as rheumatic fever in susceptible individuals with untreated group A streptococcal pharyngitis. In a previous study, we utilized a large panel of human peripheral blood cells to define the C-terminal protective epitope StreptInCor (medical identity), which does not induce autoimmune reactions. We recently confirmed the results in HLA-transgenic mice. In the present study, we extended the experimental assays to outbred animals (Swiss mice). Herein, we demonstrate high titers of StreptInCor-specific antibodies, as well as appropriate T-cell immune responses. No cross-reaction to cardiac myosin was detected. Additionally, immunized Swiss mice exhibited 87% survival one month after challenge with S. pyogenes. In conclusion, the data presented herein reinforce previous results in humans and animals and further emphasize that StreptInCor could be an effective and safe vaccine for the prevention of S. pyogenes infections.http://europepmc.org/articles/PMC3620221?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Edilberto Postol
Raquel Alencar
Fabio T Higa
Samar Freschi de Barros
Lea M F Demarchi
Jorge Kalil
Luiza Guilherme
spellingShingle Edilberto Postol
Raquel Alencar
Fabio T Higa
Samar Freschi de Barros
Lea M F Demarchi
Jorge Kalil
Luiza Guilherme
StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
PLoS ONE
author_facet Edilberto Postol
Raquel Alencar
Fabio T Higa
Samar Freschi de Barros
Lea M F Demarchi
Jorge Kalil
Luiza Guilherme
author_sort Edilberto Postol
title StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
title_short StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
title_full StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
title_fullStr StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
title_full_unstemmed StreptInCor: a candidate vaccine epitope against S. pyogenes infections induces protection in outbred mice.
title_sort streptincor: a candidate vaccine epitope against s. pyogenes infections induces protection in outbred mice.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description Infection with Streptococcus pyogenes (S. pyogenes) can result in several diseases, particularly in children. S. pyogenes M protein is the major virulence factor, and certain regions of its N-terminus can trigger autoimmune sequelae such as rheumatic fever in susceptible individuals with untreated group A streptococcal pharyngitis. In a previous study, we utilized a large panel of human peripheral blood cells to define the C-terminal protective epitope StreptInCor (medical identity), which does not induce autoimmune reactions. We recently confirmed the results in HLA-transgenic mice. In the present study, we extended the experimental assays to outbred animals (Swiss mice). Herein, we demonstrate high titers of StreptInCor-specific antibodies, as well as appropriate T-cell immune responses. No cross-reaction to cardiac myosin was detected. Additionally, immunized Swiss mice exhibited 87% survival one month after challenge with S. pyogenes. In conclusion, the data presented herein reinforce previous results in humans and animals and further emphasize that StreptInCor could be an effective and safe vaccine for the prevention of S. pyogenes infections.
url http://europepmc.org/articles/PMC3620221?pdf=render
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