Effects of LED lights on Expression of Genes Involved in Phenylpropanoid Biosynthesis and Accumulation of Phenylpropanoids in Wheat Sprout

• Main Authors: Do Manh Cuong, Tai Wook Ha, Chang Ha Park, Nam Su Kim, Hyeon Ji Yeo, Se Won Chun, Changsoo Kim, Sang Un Park
• Format: Article
• Language: English
• Published: MDPI AG 2019-06-01
• Series: Agronomy
• Subjects: wheat sprouts; phenylpropanoid; LED irradiation; gene expression
• Online Access: https://www.mdpi.com/2073-4395/9/6/307

Phenylpropanoids are naturally occurring compounds that exert beneficial pharmacological effects on human health. Phenylpropanoids can act as antioxidants and are involved in resistance to ultraviolet light and cancer; these compounds possess anti-inflammatory, antiviral, and antibacterial activity, and aid in wound healing. The expression of genes involved in phenylpropanoid biosynthesis, and consequent accumulation of phenylpropanoids in wheat sprout under conditions of stress, have not been extensively studied. This is the first study to examine the effects of light-emitting diodes (LED) on the expression of genes involved in phenylpropanoid biosynthesis and accumulation of phenylpropanoids in wheat sprouts. Our results, obtained using qRT-PCR and HPLC analyses, indicate that white light (380 nm) was the optimal wavelength for epicatechin biosynthesis in wheat sprouts. Compared with the effects of white light, blue light (470 nm) enhanced the accumulation of gallic acid and quercetin, but decreased the levels of p-coumaric acid and epicatechin; red light (660 nm) increased the accumulation of ferulic acid at 8 day and p-coumaric acid at 12 day. Compared gene expression with phenylpropanoid content showed that <i>TaPAL3</i>, <i>TaPAL4</i>, and <i>TaDFR</i> maybe important genes in phenylpropanoid biosynthesis in wheat sprout. This study provides insights into the effects of led lights on phenylpropanoid production in wheat sprouts. This knowledge will help improve secondary metabolite production in wheat sprouts.