Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis

To evaluate the promising advantages of massively parallel sequencing (MPS) in our casework, we analysed a total of 33 Y-chromosomal short tandem repeats (Y-STRs) with traditional capillary electrophoresis (CE) and 25 Y-STRs using the newer MPS technology. We studied the outcome of both technologies...

Full description

Bibliographic Details
Main Authors:	Steffi Bredemeyer, Lutz Roewer, Sascha Willuweit
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2021-03-01
Series:	Forensic Sciences Research
Subjects:	forensic sciences forensic genetics y-strs rapidly mutating y-strs sequence polymorphism isometric sequence variants massively parallel sequencing
Online Access:	http://dx.doi.org/10.1080/20961790.2021.1898078

id	doaj-01b4703122ac451e99202c917b8066a6
record_format	Article
spelling	doaj-01b4703122ac451e99202c917b8066a62021-05-06T16:05:14ZengTaylor & Francis GroupForensic Sciences Research2096-17902471-14112021-03-01001610.1080/20961790.2021.18980781898078Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresisSteffi Bredemeyer0Lutz Roewer1Sascha Willuweit2Department of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité – Universitätsmedizin BerlinDepartment of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité – Universitätsmedizin BerlinDepartment of Forensic Genetics, Institute of Legal Medicine and Forensic Sciences, Charité – Universitätsmedizin BerlinTo evaluate the promising advantages of massively parallel sequencing (MPS) in our casework, we analysed a total of 33 Y-chromosomal short tandem repeats (Y-STRs) with traditional capillary electrophoresis (CE) and 25 Y-STRs using the newer MPS technology. We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits. Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations. By detecting isometric sequence variants between unrelated males, we show that sequencing Y-STRs using MPS can boost discrimination power.http://dx.doi.org/10.1080/20961790.2021.1898078forensic sciencesforensic geneticsy-strsrapidly mutating y-strssequence polymorphismisometric sequence variantsmassively parallel sequencing
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Steffi Bredemeyer Lutz Roewer Sascha Willuweit
spellingShingle	Steffi Bredemeyer Lutz Roewer Sascha Willuweit Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis Forensic Sciences Research forensic sciences forensic genetics y-strs rapidly mutating y-strs sequence polymorphism isometric sequence variants massively parallel sequencing
author_facet	Steffi Bredemeyer Lutz Roewer Sascha Willuweit
author_sort	Steffi Bredemeyer
title	Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
title_short	Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
title_full	Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
title_fullStr	Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
title_full_unstemmed	Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis
title_sort	next generation sequencing of y-strs in father-son pairs and comparison with traditional capillary electrophoresis
publisher	Taylor & Francis Group
series	Forensic Sciences Research
issn	2096-1790 2471-1411
publishDate	2021-03-01
description	To evaluate the promising advantages of massively parallel sequencing (MPS) in our casework, we analysed a total of 33 Y-chromosomal short tandem repeats (Y-STRs) with traditional capillary electrophoresis (CE) and 25 Y-STRs using the newer MPS technology. We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits. Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations. By detecting isometric sequence variants between unrelated males, we show that sequencing Y-STRs using MPS can boost discrimination power.
topic	forensic sciences forensic genetics y-strs rapidly mutating y-strs sequence polymorphism isometric sequence variants massively parallel sequencing
url	http://dx.doi.org/10.1080/20961790.2021.1898078
work_keys_str_mv	AT steffibredemeyer nextgenerationsequencingofystrsinfathersonpairsandcomparisonwithtraditionalcapillaryelectrophoresis AT lutzroewer nextgenerationsequencingofystrsinfathersonpairsandcomparisonwithtraditionalcapillaryelectrophoresis AT saschawilluweit nextgenerationsequencingofystrsinfathersonpairsandcomparisonwithtraditionalcapillaryelectrophoresis
_version_	1721456454182895616

Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis

Similar Items