Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System

Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System

The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP). The investigated parameters involved pseudo-first-order rise and...

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Main Authors: Sayed Yahya Kazemi, Seyed Mohammad Abedirad
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/913412
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spelling doaj-01abc7b3465f48f78b417254ee0363892020-11-25T00:48:42ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/913412913412Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide SystemSayed Yahya Kazemi0Seyed Mohammad Abedirad1Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari 4818168984, IranDepartment of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari 4818168984, IranThe present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP). The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with 𝑘𝑄 values of 3.17×105 and 3.7×105M−1 in the quencher concentration range of 1.5×10−6 to 1.5×10−5 M for human serum albumin (HSA) and bovine serum albumin (BSA), respectively.http://dx.doi.org/10.1100/2012/913412
collection DOAJ
language English
format Article
sources DOAJ
author Sayed Yahya Kazemi
Seyed Mohammad Abedirad
spellingShingle Sayed Yahya Kazemi
Seyed Mohammad Abedirad
Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
The Scientific World Journal
author_facet Sayed Yahya Kazemi
Seyed Mohammad Abedirad
author_sort Sayed Yahya Kazemi
title Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
title_short Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
title_full Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
title_fullStr Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
title_full_unstemmed Effect of Human and Bovine Serum Albumin on kinetic Chemiluminescence of Mn (III)-Tetrakis (4-Sulfonatophenyl) Porphyrin-Luminol-Hydrogen Peroxide System
title_sort effect of human and bovine serum albumin on kinetic chemiluminescence of mn (iii)-tetrakis (4-sulfonatophenyl) porphyrin-luminol-hydrogen peroxide system
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2012-01-01
description The present work deals with an attempt to study the effect of human and bovine serum albumin on kinetic parameters of chemiluminescence of luminol-hydrogen peroxide system catalyzed by manganese tetrasulfonatophenyl porphyrin (MnTSPP). The investigated parameters involved pseudo-first-order rise and fall rate constant for the chemiluminescence burst, maximum level intensity, time to reach maximum intensity, total light yield, and values of the intensity at maximum CL which were evaluated by nonlinear least square program KINFIT. Because of interaction of metalloporphyrin with proteins, the CL parameters are drastically affected. The systems resulted in Stern-Volmer plots with 𝑘𝑄 values of 3.17×105 and 3.7×105M−1 in the quencher concentration range of 1.5×10−6 to 1.5×10−5 M for human serum albumin (HSA) and bovine serum albumin (BSA), respectively.
url http://dx.doi.org/10.1100/2012/913412
work_keys_str_mv AT sayedyahyakazemi effectofhumanandbovineserumalbuminonkineticchemiluminescenceofmniiitetrakis4sulfonatophenylporphyrinluminolhydrogenperoxidesystem
AT seyedmohammadabedirad effectofhumanandbovineserumalbuminonkineticchemiluminescenceofmniiitetrakis4sulfonatophenylporphyrinluminolhydrogenperoxidesystem
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