Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Whole-genome sequencing had greater ab...

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Bibliographic Details
Main Authors: Tsuyoshi Sekizuka, Chihiro Katsukawa, Makoto Kuroda, Keigo Shibayama, Ken Otsuji, Mitsumasa Saito, Akihiko Yamamoto, Masaaki Iwaki
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2020-10-01
Series:Emerging Infectious Diseases
Subjects:
Corynebacterium ulcerans
molecular typing
genome sequence
multilocus sequence typing
phylogeny
bacteria
Online Access:https://wwwnc.cdc.gov/eid/article/26/10/20-0086_article
Description
Summary:We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate’s ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Whole-genome sequencing had greater ability to discriminate lineages at high resolution.
ISSN:1080-6040
1080-6059

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