Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology

Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The i...

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Main Authors: Wataru Koto, Yoshinori Shinohara, Kazuyuki Kitamura, Takanori Wachi, Seicho Makihira, Kiyoshi Koyano
Format: Article
Language:English
Published: MDPI AG 2017-10-01
Series:Nanomaterials
Subjects:
Online Access:https://www.mdpi.com/2079-4991/7/10/322
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spelling doaj-016d517a632847f19c99440100e397e32020-11-25T01:05:47ZengMDPI AGNanomaterials2079-49912017-10-0171032210.3390/nano7100322nano7100322Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic NanotechnologyWataru Koto0Yoshinori Shinohara1Kazuyuki Kitamura2Takanori Wachi3Seicho Makihira4Kiyoshi Koyano5Section of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanSection of Fixed Prosthodontics, Department of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, JapanMagnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial–mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.https://www.mdpi.com/2079-4991/7/10/322magnetic nanoparticlesnanotechnologycell sheetodontogenic cellsepithelial-mesenchymal interactionsdental enamel regeneration
collection DOAJ
language English
format Article
sources DOAJ
author Wataru Koto
Yoshinori Shinohara
Kazuyuki Kitamura
Takanori Wachi
Seicho Makihira
Kiyoshi Koyano
spellingShingle Wataru Koto
Yoshinori Shinohara
Kazuyuki Kitamura
Takanori Wachi
Seicho Makihira
Kiyoshi Koyano
Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
Nanomaterials
magnetic nanoparticles
nanotechnology
cell sheet
odontogenic cells
epithelial-mesenchymal interactions
dental enamel regeneration
author_facet Wataru Koto
Yoshinori Shinohara
Kazuyuki Kitamura
Takanori Wachi
Seicho Makihira
Kiyoshi Koyano
author_sort Wataru Koto
title Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
title_short Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
title_full Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
title_fullStr Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
title_full_unstemmed Porcine Dental Epithelial Cells Differentiated in a Cell Sheet Constructed by Magnetic Nanotechnology
title_sort porcine dental epithelial cells differentiated in a cell sheet constructed by magnetic nanotechnology
publisher MDPI AG
series Nanomaterials
issn 2079-4991
publishDate 2017-10-01
description Magnetic nanoparticles (MNPs) are widely used in medical examinations, treatments, and basic research, including magnetic resonance imaging, drug delivery systems, and tissue engineering. In this study, MNPs with magnetic force were applied to tissue engineering for dental enamel regeneration. The internalization of MNPs into the odontogenic cells was observed by transmission electron microscopy. A combined cell sheet consisting of dental epithelial cells (DECs) and dental mesenchymal cells (DMCs) (CC sheet) was constructed using magnetic force-based tissue engineering technology. The result of the iron staining indicated that MNPs were distributed ubiquitously over the CC sheet. mRNA expression of enamel differentiation and basement membrane markers was examined in the CC sheet. Immunostaining showed Collagen IV expression at the border region between DEC and DMC layers in the CC sheet. These results revealed that epithelial–mesenchymal interactions between DEC and DMC layers were caused by bringing DECs close to DMCs mechanically by magnetic force. Our study suggests that the microenvironment in the CC sheet might be similar to that during the developmental stage of a tooth bud. In conclusion, a CC sheet employing MNPs could be developed as a novel and unique graft for artificially regenerating dental enamel.
topic magnetic nanoparticles
nanotechnology
cell sheet
odontogenic cells
epithelial-mesenchymal interactions
dental enamel regeneration
url https://www.mdpi.com/2079-4991/7/10/322
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AT yoshinorishinohara porcinedentalepithelialcellsdifferentiatedinacellsheetconstructedbymagneticnanotechnology
AT kazuyukikitamura porcinedentalepithelialcellsdifferentiatedinacellsheetconstructedbymagneticnanotechnology
AT takanoriwachi porcinedentalepithelialcellsdifferentiatedinacellsheetconstructedbymagneticnanotechnology
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AT kiyoshikoyano porcinedentalepithelialcellsdifferentiatedinacellsheetconstructedbymagneticnanotechnology
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