Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors

Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone...

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Main Authors:	Pavel Řezanka, Denisa Macková, Radek Jurok, Michal Himl, Martin Kuchař
Format:	Article
Language:	English
Published:	MDPI AG 2020-06-01
Series:	Molecules
Subjects:	capillary electrophoresis chiral separation cyclodextrin mephedrone metabolites
Online Access:	https://www.mdpi.com/1420-3049/25/12/2879

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spelling	doaj-012b436def3840b0b561d713d79d09c22020-11-25T03:47:05ZengMDPI AGMolecules1420-30492020-06-01252879287910.3390/molecules25122879Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral SelectorsPavel Řezanka0Denisa Macková1Radek Jurok2Michal Himl3Martin Kuchař4Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Chemistry of Natural Compounds, Forensic Laboratory of Biologically Active Substances, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Organic Chemistry, Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Chemistry of Natural Compounds, Forensic Laboratory of Biologically Active Substances, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 5, 166 28 Prague 6, Czech RepublicMephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L<sup>−1</sup> carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L<sup>−1</sup>; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel’s fitting test, the linear calibration ranges were determined.https://www.mdpi.com/1420-3049/25/12/2879capillary electrophoresischiral separationcyclodextrinmephedronemetabolites
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Pavel Řezanka Denisa Macková Radek Jurok Michal Himl Martin Kuchař
spellingShingle	Pavel Řezanka Denisa Macková Radek Jurok Michal Himl Martin Kuchař Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors Molecules capillary electrophoresis chiral separation cyclodextrin mephedrone metabolites
author_facet	Pavel Řezanka Denisa Macková Radek Jurok Michal Himl Martin Kuchař
author_sort	Pavel Řezanka
title	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors
title_short	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors
title_full	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors
title_fullStr	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors
title_full_unstemmed	Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors
title_sort	enantioseparation and determination of mephedrone and its metabolites by capillary electrophoresis using cyclodextrins as chiral selectors
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2020-06-01
description	Mephedrone, a psychoactive compound derived from cathinone, is widely used as a designer drug. The determination of mephedrone and its metabolites is important for understanding its possible use in medicine. In this work, a method of capillary electrophoresis for the chiral separation of mephedrone and its metabolites was developed. Carboxymethylated β-cyclodextrin was selected as the most effective chiral selector from seven tested cyclodextrin derivates. Based on the simplex method, the optimal composition of the background electrolyte was determined: at pH 2.75 and 7.5 mmol·L<sup>−1</sup> carboxymethylated β-cyclodextrin the highest total resolution of a mixture of analytes was achieved. For mephedrone and its metabolites, calibration curves were constructed in a calibration range from 0.2 to 5 mmol·L<sup>−1</sup>; limits of detection, limits of quantification, precision, and repeatability were calculated, and according to Mandel’s fitting test, the linear calibration ranges were determined.
topic	capillary electrophoresis chiral separation cyclodextrin mephedrone metabolites
url	https://www.mdpi.com/1420-3049/25/12/2879
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Enantioseparation and Determination of Mephedrone and Its Metabolites by Capillary Electrophoresis Using Cyclodextrins as Chiral Selectors

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