In vitro evaluation of cytotoxicity and corrosion behavior of commercially pure titanium and Ti-6Al-4V alloy for dental implants

• Main Authors: Sanchitha Chandar, Ravindra Kotian, Prashanthi Madhyastha, Shama Prasada Kabekkodu, Padmalatha Rao
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017-01-01
• Series: The Journal of Indian Prosthodontic Society
• Subjects: Artificial saliva; corrosion; cytotoxicity; gingival fibroblast; 3-(4; 5-dimethylthiazol-2-yl)-2; 5-diphenyltetrazolium bromide assay; scanning electron microscopy; Ti-6Al-4V; titanium
• Online Access: http://www.j-ips.org/article.asp?issn=0972-4052;year=2017;volume=17;issue=1;spage=35;epage=40;aulast=Chandar
• ISSN: 0972-4052; 1998-4057

Purpose: The aim of this study was to investigate the cytotoxicity in human gingival fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and corrosion behavior by potentiodynamic polarization technique of commercially pure titanium (Ti 12) and its alloy Ti-6Al-4V (Ti 31). Materials and Methods: In the present in vitro study, cytotoxicity of Ti 12 and Ti 31 in human gingival fibroblast by MTT assay and the corrosion behavior by potentiodynamic polarization technique in aqueous solutions of 0.1 N NaCl, 0.1 N KCl, and artificial saliva with and without NaF were studied. Results: The independent t-test within materials and paired t-test with time interval showed higher cell viability for Ti 12 compared to Ti 31. Over a period, cell viability found to stabilize in both Ti 12 and Ti 31. The effects of ions of Ti and alloying elements aluminum and vanadium on the cell viability were found with incubation period of cells on samples to 72 h. The electrochemical behavior of Ti 12 and Ti 31 in different experimental solutions showed a general tendency for the immersion potential to shift steadily toward nobler values indicated formation of TiO2and additional metal oxides. The multiphase alloy Ti-6Al-4V showed more surface pitting. Conclusion: The commercially pure Ti showed better cell viability compared to Ti 31. Less cell viability in Ti 31 is because of the presence of aluminum and vanadium. A significant decrease in cytotoxicity due to the formation of TiO2over a period of time was observed both in Ti 12 and Ti 31. The electrochemical behavior of Ti 12 and Ti 31 in different experimental solutions showed a general tendency for the immersion potential to shift steadily toward nobler values indicated formation of TiO2 and additional metal oxides. Ti 31 alloy showed surface pitting because of its multiphase structure.