Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka

Evaluation of rapid extraction and isothermal amplification techniques for the detection of Leishmania donovani DNA from skin lesions of suspected cases at the point of need in Sri Lanka

Abstract Background Leishmaniasis is a disease caused by vector-borne protozoans. In Sri Lanka, the cutaneous form of the disease is predominant, which is usually diagnosed using Giemsa-stained slit skin smear examination and by histology. However, the sensitivity of slit skin smears and histology a...

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Main Authors: Gayana Gunaratna, Aresha Manamperi, Susanne Böhlken-Fascher, Renu Wickremasinge, Kithsiri Gunawardena, Bandujith Yapa, Nishantha Pathirana, Hasantha Pathirana, Nilanthi de Silva, Monica Sooriyaarachchi, Theja Deerasinghe, Dinesh Mondal, Shalindra Ranasinghe, Ahmed Abd El Wahed
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Parasites & Vectors
Subjects:
Recombinase polymerases amplification
Leishmania donovani
Point of need
Molecular assay
Online Access:http://link.springer.com/article/10.1186/s13071-018-3238-1

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