Further Evidence of Possible Therapeutic Uses of Sambucus nigra L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

Sambucus nigra L. is widely used in traditional medicine with different applications. However, confirmative studies are strongly required. This study aimed to assess the biological activities of the S. nigra flower’s extract encapsulated into two different types...

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Main Authors:	Ana Henriques Mota, Noélia Duarte, Ana Teresa Serra, António Ferreira, Maria Rosário Bronze, Luísa Custódio, Maria Manuela Gaspar, Sandra Simões, Patrícia Rijo, Lia Ascensão, Pedro Faísca, Ana Silveira Viana, Rui Pinto, Pradeep Kumar, António José Almeida, Catarina Pinto Reis
Format:	Article
Language:	English
Published:	MDPI AG 2020-12-01
Series:	Pharmaceutics
Subjects:	<i>Sambucus nigra</i> L. nanoparticles anti-inflammatory activity collagenase inhibition safety assessment
Online Access:	https://www.mdpi.com/1999-4923/12/12/1181

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record_format	Article
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ana Henriques Mota Noélia Duarte Ana Teresa Serra António Ferreira Maria Rosário Bronze Luísa Custódio Maria Manuela Gaspar Sandra Simões Patrícia Rijo Lia Ascensão Pedro Faísca Ana Silveira Viana Rui Pinto Pradeep Kumar António José Almeida Catarina Pinto Reis
spellingShingle	Ana Henriques Mota Noélia Duarte Ana Teresa Serra António Ferreira Maria Rosário Bronze Luísa Custódio Maria Manuela Gaspar Sandra Simões Patrícia Rijo Lia Ascensão Pedro Faísca Ana Silveira Viana Rui Pinto Pradeep Kumar António José Almeida Catarina Pinto Reis Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations Pharmaceutics <i>Sambucus nigra</i> L. nanoparticles anti-inflammatory activity collagenase inhibition safety assessment
author_facet	Ana Henriques Mota Noélia Duarte Ana Teresa Serra António Ferreira Maria Rosário Bronze Luísa Custódio Maria Manuela Gaspar Sandra Simões Patrícia Rijo Lia Ascensão Pedro Faísca Ana Silveira Viana Rui Pinto Pradeep Kumar António José Almeida Catarina Pinto Reis
author_sort	Ana Henriques Mota
title	Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
title_short	Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
title_full	Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
title_fullStr	Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
title_full_unstemmed	Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations
title_sort	further evidence of possible therapeutic uses of <i>sambucus nigra</i> l. extracts by the assessment of the in vitro and in vivo anti-inflammatory properties of its plga and pcl-based nanoformulations
publisher	MDPI AG
series	Pharmaceutics
issn	1999-4923
publishDate	2020-12-01
description	<i>Sambucus nigra</i> L. is widely used in traditional medicine with different applications. However, confirmative studies are strongly required. This study aimed to assess the biological activities of the <i>S. nigra</i> flower’s extract encapsulated into two different types of nanoparticles for optimizing its properties and producing further evidence of its potential therapeutic uses. Different nanoparticles (poly(lactide-co-glycolide, PLGA) and poly-Ɛ-caprolactone (PCL), both with oleic acid, were prepared by emulsification/solvent diffusion and solvent-displacement methods, respectively. Oleic acid was used as a capping agent. After the nanoparticles’ preparation, they were characterized and the biological activities were studied in terms of collagenase, in vitro and in vivo anti-inflammatory, and in vitro cell viability. Rutin and naringenin were found to be the major phenolic compounds in the studied extract. The encapsulation efficiency was higher than 76% and revealed to have an impact on the release of the extract, mainly for the PLGA. Moreover, biochemical and histopathological analyses confirmed that the extract-loaded PLGA-based nanoparticles displayed the highest anti-inflammatory activity. In addition to supporting the previously reported evidence of potential therapeutic uses of <i>S. nigra</i>, these results could draw the pharmaceutical industry’s interest to the novelty of the nanoproducts.
topic	<i>Sambucus nigra</i> L. nanoparticles anti-inflammatory activity collagenase inhibition safety assessment
url	https://www.mdpi.com/1999-4923/12/12/1181
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spelling	doaj-0102c70fe1dc4a29b097641d95ccef742020-12-05T00:04:41ZengMDPI AGPharmaceutics1999-49232020-12-01121181118110.3390/pharmaceutics12121181Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based NanoformulationsAna Henriques Mota0Noélia Duarte1Ana Teresa Serra2António Ferreira3Maria Rosário Bronze4Luísa Custódio5Maria Manuela Gaspar6Sandra Simões7Patrícia Rijo8Lia Ascensão9Pedro Faísca10Ana Silveira Viana11Rui Pinto12Pradeep Kumar13António José Almeida14Catarina Pinto Reis15iMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalIBET, Instituto de Biologia Experimental e Tecnológica, Av. da República, Estação Agronómica, Apartado 12, 2780-901 Oeiras, PortugalIBET, Instituto de Biologia Experimental e Tecnológica, Av. da República, Estação Agronómica, Apartado 12, 2780-901 Oeiras, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalCentre of Marine Sciences, Faculty of Sciences and Technology, University of Algarve, Ed. 7, Campus of Gambelas, 8005-139 Faro, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalCentro de Estudos do Ambiente e do Mar (CESAM), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugalFaculdade de Medicina Veterinária—Universidade Lusófona de Humanidades e Tecnologias/DNAtech Laboratório Veterinário, Campo Grande 376, 1749-024 Lisboa, PortugalCentro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugalDepartment of Pharmacy and Pharmacology, School of Therapeutic Sciences, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown 2193, South AfricaiMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, PortugaliMED, ULisboa, Research Institute for Medicines, Faculdade de Farmácia, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003 Lisboa, Portugal<i>Sambucus nigra</i> L. is widely used in traditional medicine with different applications. However, confirmative studies are strongly required. This study aimed to assess the biological activities of the <i>S. nigra</i> flower’s extract encapsulated into two different types of nanoparticles for optimizing its properties and producing further evidence of its potential therapeutic uses. Different nanoparticles (poly(lactide-co-glycolide, PLGA) and poly-Ɛ-caprolactone (PCL), both with oleic acid, were prepared by emulsification/solvent diffusion and solvent-displacement methods, respectively. Oleic acid was used as a capping agent. After the nanoparticles’ preparation, they were characterized and the biological activities were studied in terms of collagenase, in vitro and in vivo anti-inflammatory, and in vitro cell viability. Rutin and naringenin were found to be the major phenolic compounds in the studied extract. The encapsulation efficiency was higher than 76% and revealed to have an impact on the release of the extract, mainly for the PLGA. Moreover, biochemical and histopathological analyses confirmed that the extract-loaded PLGA-based nanoparticles displayed the highest anti-inflammatory activity. In addition to supporting the previously reported evidence of potential therapeutic uses of <i>S. nigra</i>, these results could draw the pharmaceutical industry’s interest to the novelty of the nanoproducts.https://www.mdpi.com/1999-4923/12/12/1181<i>Sambucus nigra</i> L.nanoparticlesanti-inflammatory activitycollagenase inhibitionsafety assessment

Further Evidence of Possible Therapeutic Uses of <i>Sambucus nigra</i> L. Extracts by the Assessment of the In Vitro and In Vivo Anti-Inflammatory Properties of Its PLGA and PCL-Based Nanoformulations

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