Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices

Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices

Summary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells. The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS. Here, we describe a methodology...

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Main Authors: Chirag Vasavda, Solomon H. Snyder, Bindu D. Paul
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell biology
Model organisms
Molecular biology
Neuroscience
Molecular/chemical probe
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000393
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spelling doaj-00da5ccbf59f4a5aaf526367e3217fea2021-03-22T12:53:17ZengElsevierSTAR Protocols2666-16672021-03-0121100332Quantitative measurement of reactive oxygen species in ex vivo mouse brain slicesChirag Vasavda0Solomon H. Snyder1Bindu D. Paul2The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Corresponding authorThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USAThe Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Corresponding authorSummary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells. The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS. Here, we describe a methodology to quantitatively measure ROS in ex vivo mouse brain slices at baseline and after neural stimulation. Evaluating ROS in slices provides a more complete picture of neural redox signaling than when measured in isolated neurons or astrocytes.For complete details on the use and execution of this protocol, please refer to Vasavda et al. (2019).http://www.sciencedirect.com/science/article/pii/S2666166721000393Cell biologyModel organismsMolecular biologyNeuroscienceMolecular/chemical probe
collection DOAJ
language English
format Article
sources DOAJ
author Chirag Vasavda
Solomon H. Snyder
Bindu D. Paul
spellingShingle Chirag Vasavda
Solomon H. Snyder
Bindu D. Paul
Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
STAR Protocols
Cell biology
Model organisms
Molecular biology
Neuroscience
Molecular/chemical probe
author_facet Chirag Vasavda
Solomon H. Snyder
Bindu D. Paul
author_sort Chirag Vasavda
title Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
title_short Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
title_full Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
title_fullStr Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
title_full_unstemmed Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
title_sort quantitative measurement of reactive oxygen species in ex vivo mouse brain slices
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2021-03-01
description Summary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells. The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS. Here, we describe a methodology to quantitatively measure ROS in ex vivo mouse brain slices at baseline and after neural stimulation. Evaluating ROS in slices provides a more complete picture of neural redox signaling than when measured in isolated neurons or astrocytes.For complete details on the use and execution of this protocol, please refer to Vasavda et al. (2019).
topic Cell biology
Model organisms
Molecular biology
Neuroscience
Molecular/chemical probe
url http://www.sciencedirect.com/science/article/pii/S2666166721000393
work_keys_str_mv AT chiragvasavda quantitativemeasurementofreactiveoxygenspeciesinexvivomousebrainslices
AT solomonhsnyder quantitativemeasurementofreactiveoxygenspeciesinexvivomousebrainslices
AT bindudpaul quantitativemeasurementofreactiveoxygenspeciesinexvivomousebrainslices
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