Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices

Quantitative measurement of reactive oxygen species in ex vivo mouse brain slices

Summary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells. The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS. Here, we describe a methodology...

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Bibliographic Details
Main Authors: Chirag Vasavda, Solomon H. Snyder, Bindu D. Paul
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell biology
Model organisms
Molecular biology
Neuroscience
Molecular/chemical probe
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000393
Description
Summary:Summary: Evaluating redox homeostasis involves gauging the levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) directly in tissues and cells. The brain is especially metabolically active and is particularly vulnerable to excessive ROS and RNS. Here, we describe a methodology to quantitatively measure ROS in ex vivo mouse brain slices at baseline and after neural stimulation. Evaluating ROS in slices provides a more complete picture of neural redox signaling than when measured in isolated neurons or astrocytes.For complete details on the use and execution of this protocol, please refer to Vasavda et al. (2019).
ISSN:2666-1667

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