Determination of Cholesterol Content in Butter by HPLC: Up-to-Date Optimization, and In-House Validation Using Reference Materials

This work deals with up-to-date optimization of cholesterol content determination when saponification and extraction procedures as well as HPLC conditions were studied. As found, optimal conditions for saponification process were identified by 15 min heating in the presence of 0.015 L of methanolic...

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Bibliographic Details
Main Authors: Lukáš Kolarič, Peter Šimko
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/9/10/1378