A rapid and sensitive radiochemical assay for phosphatidic acid phosphohydrolase activity
A technique is described for the radiochemical assay of phosphatidic acid phosphohydrolase activity in rat brain. Radiochemically pure 32P-labeled phosphatidic acid of known specific radioactivity and structure, which was biosynthesized in vitro by the diacylglycerol kinase of E. coli, was used as t...
Main Authors: | T J Flynn, D S Deshmukh, R A Pieringer |
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Format: | Article |
Language: | English |
Published: |
Elsevier
1977-11-01
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Series: | Journal of Lipid Research |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520415974 |
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